elisareagents

A uniform quantitative enzyme-linked immunosorbent assay for Coxsackievirus A16 antigen in vaccine

Coxsackievirus A16 (CV-A16), considered one of main etiological brokers of hand, foot and mouth illness (HFMD), causes outbreaks of the illness in younger youngsters everywhere in the world. In an effort to promote the prevention and management of HFMD, the analysis and growth of CV-A16 vaccine have been carried out in China.
  • Nevertheless, on account of missing of a acknowledged CV-A16 antigen detection methodology, the analysis and high quality management (QC) of vaccine effectiveness are tremendously restricted. On this research, we established a quantitative enzyme-linked immunosorbent assay (Q-ELISA) to find out the antigen focus in CV-A16 vaccines that may be utilized in manufacturing in China.
  • A neutralizing antibody 16E1 was used as a seize antibody that may bind to varied CV-A16 antigens of various subgenotypes, and an antiserum from CV-A16-immunized rabbit conjugated by HRP was appropriate for detecting and quantifying CV-A16 antigens. The Q-ELISA was validated for specificity, linearity, accuracy, precision and robustness through the use of the CV-A16 antigen nationwide commonplace (NS).
  • Moreover, we utilized the Q-ELISA to quantify antigen contents of vaccine bulks from six producers and different intermediate merchandise from one producer. The outcomes indicated that the Q-ELISA can fulfill the necessities of QC for all producers concerned.
Key phrases: Coxsackievirus A16 (CV-A16); Q-ELISA; antigen; neutralizing antibody; vaccine

Consistency analysis of human serum 25-hydroxyvitamin D utilizing liquid chromatography-tandem mass spectrometry and enzyme-linked immunosorbent assay

Goal: Comparability of liquid chromatography-tandem mass spectrometry(LC-MS/MS) and enzyme-linked immunosorbent assay(ELISA) for the detection of 25(OH)D focus in human serum and the analysis of vitamin D deficiency.
Strategies: Within the serum pool of "Nationwide Institute for Vitamin and Well being, Chinese language Heart for Illness Management and Prevention", 500 serum samples of ladies aged 18 to 45 years previous had been randomly chosen, and 25(OH)D ranges had been measured by ELISA and LC-MS/MS for a similar serum pattern, respectively. The LC-MS/MS column was Waters XBridge BEH C_(18)(2. 1 mm×50 mm, 2. 5 μm).

The correlation between the 2 methodology was examined by correlation evaluation, regression evaluation and consistency check. The Endocrine Society and Institute of Drugs suggestions had been used to find out the deficiency of vitamin D, and the McNemar check, Kappa coefficient and diagnostic check had been used to diagnose the consistency of vitamin D deficiency.

Outcomes: The regression equation for the 25(OH)D focus measured by the 2 methodology was y_(LC-MS/MS)=-0. 035+1. 007×x_(ELISA)(r=0. 877), and the typical deviation between the 2 was 4. 48% and the intraclass correlation coefficient was 0. 87. The 25(OH)D focus was lower than 12 and 20 ng/mL as a criterion for vitamin D deficiency, and the Kappa coefficients had been higher than 0. 60(0. 64 and 0. 74).

Conclusion: When serum 25(OH)D stage was detected by LC-MS/MS and ELISA, the correspondence of the 2 methodology was wonderful. Taking the “gold commonplace” LC-MS/MS methodology as a reference, the ELISA methodology was used to find out human vitamin D deficiency with good sensitivity and specificity, which can be utilized for the correct and speedy detection of large-scale inhabitants samples.

Key phrases: 25(OH)D; blood check; enzyme-linked immunosorbent assay; liquid chromatography-tandem mass spectrometry.
elisareagents
elisareagents

96 well filter plate (960ul each well)

SD5006 12UNIT
EUR 96.54

2 x Clear 96 well Plate, 2EA

X003-2EA 2EA
EUR 65

2 x Clear 96 well Plate, 2EA

X018-2EA 2EA
EUR 65

96 WELL CLEAR PCR PLATE FOR ABI

PCR-96-AB-C 10/pk
EUR 167
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY

P-DW-12-HC 5/pk
EUR 152
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

96 well filter plate (no filter)

SD5006-EMPTY 12UNIT
EUR 87.41

Polystyrene-bottomed 96-well plate (ELISA)

CT361 6
EUR 73

Clear Coated 96 Well Plate, 1EA

C107-1EA 1EA
EUR 189

96-Well White Plates

M1354-2
EUR 131

96-Well Black Plates

M1355-2
EUR 131

96 WELL CLEAR PCR HALF SKIRT AMPLIFICATION PLATE

PCR-96-HS-C 10/pk
EUR 159
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96 WELL SITTING DROP HIGH THROUGHPUT CRYSTALLOGRAPHY PLATE.

CP-AXYGEM-96-50 10/pk
EUR 457
Description: Crystallography Plates; Crystallography Plates - Axygen

96 well DNA plate with membrane (960ul each well)

SD5007 12UNIT
EUR 242.7

96 well RNA plate with membrane (960ul each well)

SD5009 12UNIT
EUR 260.97

96-Well Plate PCR Products Purification Kit

BS365 5XPlates, 480prep
EUR 250.31

96-Well Plate PCR Products Purification Kit

BS3652 2xPlates, 192prep
EUR 155.16

96-Well Plate DNA Cleanup Miniprep Kit

BS369 5XPlates, 480prep
EUR 250.31

96-Well Plate DNA Cleanup Miniprep Kit

BS3692 2xPlates, 192prep
EUR 155.16

96-Well Plate Plasmid DNA Miniprep Kit

BS415 5XPlates, 480prep
EUR 250.31

96-Well Plate Plasmid DNA Miniprep Kit

BS4152 2XPlates, 192prep
EUR 155.16

96-Well Plate Viral DNA Miniprep Kit

VT92032 2XPlates, 192prep
EUR 413.22

Polystyrene-bottomed 96-well plate + lid (ELISPOT)

CT365 5
EUR 78

5 x Clear 96 well Plate, 5EA

X003-5EA 5EA
EUR 80

4 x Clear 96 Well Plate, 4EA

X018-4EA 4EA
EUR 87

Black Half Area 96 Well Plate, 1EA

X023-1EA 1EA
EUR 80

96 Well Breast Tumor Genomic DNA Plate

D8235086-1 1 plate
EUR 316

96 Well Colon Tumor Genomic DNA Plate

D8235090-1 1 plate
EUR 316

96 Well Lung Tumor Genomic DNA Plate

D8235152-1 1 plate
EUR 316

96 Well Stomach Tumor Genomic DNA Plate

D8235248-1 1 plate
EUR 316

2 x Black Half Area 96 Well Plate, 2EA

X037-2EA 2EA
EUR 94

96 Well Half-Area Microplates

M1356-2 2 Plates
EUR 146

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY PRE-STERILIZED

P-DW-12-HC-S 5/pk
EUR 207
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

SpotLiter? 96 well Plate Light Tracker (foot pedal & USB included)

ACT-SL-96
EUR 647

96-well Plasmid DNA Extraction Kit (1 plate)

FAPWE-96001 1 prep
EUR 146

96 Well Plate RNA Cleanup and Concentration Kit

BRC1251 2xPlates, 192prep
EUR 250.31

96-Well Plate Animal Genomic DNA Miniprep Kit

BS437 5XPlates, 480prep
EUR 362.98

96-Well Plate Animal Genomic DNA Miniprep Kit

BS4372 2XPlates, 192prep
EUR 195.5

96 Well Plate Yeast Genomic DNA Miniprep Kit

BS8357 2XPlates, 192prep
EUR 195.5

96-Well Plate Plant Genomic DNA Miniprep Kit

BS8361 2XPlates, 192prep
EUR 195.5

96 Well Plate Blood Genomic DNA Miniprep Kit

BT92031 2Plates, 192prep
EUR 175.71

96 Well Plate Blood Genomic DNA Miniprep Kit

BT92032 5Plates, 480prep
EUR 324.92

Block for 96 well plate, WSC-2620/30

4002659 1unit
EUR 382
Description: Bl ock for WSC-2620 / 2630

96-Well Plate Bacteria Genomic DNA Miniprep Kit

SK1292 2XPlates, 192prep
EUR 175.71

96-Well Plate Bacteria Genomic DNA Miniprep Kit

SK1295 5XPlates, 480prep
EUR 324.92

PVDF membrane-bottomed 96-well plate + lid (ELISPOT)

CT366 5
EUR 224

96 Well ExoELISA plate (12x8 well strips, pack of 10 plates)

EXOEL-96P-1 10 Plates
EUR 213

Progesterone EIA kit (One Plate)

K025-H1 1x96 well plate
EUR 305

Estradiol EIA kit (One Plate)

K030-H1 1x96 well plate
EUR 283

Estrone EIA kit (One Plate)

K031-H1 1x96 well plate
EUR 300

Testosterone EIA kit (One Plate)

K032-H1 1x96 well plate
EUR 316

Prolactin EIA kit (One Plate)

K040-H1 1x96 well plate
EUR 471

Allopregnanolone EIA Kit (One Plate)

K044-H1 1x96 well plate
EUR 425

Allopregnanolone EIA Kit (Five Plate)

K044-H5 5x96 well plate
EUR 1490

Insulin EIA Kit (One Plate)

K046-H1 1x96 well plate
EUR 501

Oxytocin EIA Kit (One Plate)

K048-H1 1x96 well plate
EUR 450

Oxytocin EIA Kit (Five Plate)

K048-H5 5x96 well plate
EUR 1597

Aldosterone EIA Kit (One Plate)

K052-H1 1x96 well plate
EUR 387

Aldosterone EIA Kit (Five Plate)

K052-H5 5x96 well plate
EUR 1355

Epiandrosterone EIA Kit (One Plate)

K063-H1 1x96 well plate
EUR 436

Epiandrosterone EIA Kit (Five Plate)

K063-H5 5x96 well plate
EUR 1545

Estriol EIA Kit (One Plate)

K064-H1 1x96 well plate
EUR 327

Estriol EIA Kit (Five Plate)

K064-H5 5x96 well plate
EUR 1110

Adaptor for 96-well PCR plate, accessory for AgileSealer?

APS-200-96PCR
EUR 351

96 Well Plate Bacterial Total RNA Miniprep Super Kit

BS585 5XPlates, 480prep
EUR 487.82

96 Well Plate Bacterial Total RNA Miniprep Super Kit

BS5852 2XPlates, 192prep
EUR 250.31

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit

BS92028 2XPlates, 192prep
EUR 212.25

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit

BS92029 5XPlates, 480prep
EUR 364.5

Block for 96 deep-well plate, WSC-2620/30

4002661 1unit
EUR 382
Description: Bl ock for WSC-2620 / 2630

COOLSINK XT 96U, 96 WELL U-BOTTOM PLATE MODULE

432071 1/pk
EUR 345
Description: BioCision; BioCision CoolBox

SINGLE BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED

480130 1/pk
EUR 223
Description: Lab Equipment; Constant Temperature Equipment

DUAL BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED

480132 1/pk
EUR 218
Description: Lab Equipment; Constant Temperature Equipment

96 Well PCR Plates, Fits ABI Fast Plate Machines

GP615 25 plates
EUR 212

Mouse anti-Osteopontin Clear Coated 96 Well Plate, 1EA

C075-1EA 1EA
EUR 178

Mouse anti-Prolactin Clear Coated 96 Well Plate, 1EA

C140-1EA 1EA
EUR 178

Mouse anti-Myeloperoxidase Clear Coated 96 Well Plate, 1EA

C223-1EA 1EA
EUR 178

Goat anti-Mouse, Coated White 96 Well Plate, 1EA

X010-1EA 1EA
EUR 109

Goat anti-Rabbit, Coated White 96 Well Plate, 1EA

X014-1EA 1EA
EUR 109

Goat anti-Rabbit, Coated Clear 96 Well Plate, 1EA

X016-1EA 1EA
EUR 109

Donkey anti-Sheep, Coated Clear 96 Well Plate, 1EA

X061-1EA 1EA
EUR 109

Donkey anti-Sheep, Coated White 96 Well Plate, 1EA

X063-1EA 1EA
EUR 109

96 WELL BARCODED H1-H12, CLEAR PCR PLATE FOR ABI, 0,2ML

PCR-96-AB-C-PBC 10/pk
EUR 195
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

Analysis of human and canine Brucella canis an infection: growth and analysis of oblique enzyme-linked immunosorbent assays utilizing recombinant Brucella proteins.

Brucella canis, a Gram-negative coccobacilli belonging to the genus Brucellae, is a pathogenic bacterium that may produce infections in canines and people. A number of research have been carried out to develop diagnostic methods to detect all zoonotic Brucellae. Analysis of Brucella canis an infection is difficult as a result of lack of extremely particular and delicate diagnostic assays.

This work was divided in two phases: within the first one, had been recognized antigenic proteins in B. canis that would probably be used for serological analysis of brucellosis. Human sera optimistic for canine brucellosis an infection was used to acknowledge immunoreactive proteins that had been then recognized by performing 2D-GEL and immunoblot assays.

These spots had been analyzed utilizing MALDI TOF MS and predicted proteins had been recognized. Of the 35 protein spots analyzed, 14 proteins had been recognized and subsequently characterised utilizing bioinformatics, two of this had been chosen for the following section. Within the second section, we developed and validated an oblique enzyme-linked immunosorbent assays utilizing these recombinant proteins: inosine 5′ phosphate dehydrogenase, pyruvate dehydrogenase E1 subunit beta (PdhB) and elongation issue Tu (Tuf).

These genes had been PCR-amplified from genomic DNA of B. canis pressure Oliveri, cloned, and expressed in Escherichia coli. Recombinant proteins had been purified by metallic affinity chromatography, and used as antigens in oblique ELISA. Serum samples from wholesome and B. canis-infected people and canines had been used to guage the efficiency of oblique ELISAs. Our outcomes counsel that PdhB and Tuf proteins could possibly be used as antigens for serologic detection of B. canis an infection in people, however not in canines.

The usage of recombinant antigens in iELISA assays to detect B. canis-specific antibodies in human serum could possibly be a beneficial instrument to enhance analysis of human brucellosis attributable to B. canis.

Key phrases: Micro organism; Bacteriology; Organic sciences; Brucella canis str. Oliveri; Brucellosis; ELISA check; Well being sciences; Immunoproteome: people; Infectious illness; Microbiology; Microorganism; Proteins; Proteomics; Veterinary drugs; Zoonoses.

EZBlock? (PBS) Blocking Buffer

2128-1000
EUR 229

EZBlock? (PBS) Blocking Buffer

2128-200
EUR 137

Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed

ELISA-1 1
EUR 202

Blocking Buffer and Coating Stabilizer

abx090811-1000ml 1000 ml
EUR 523

West-Ezier Super Blocking Buffer

W3700-010 100ml
EUR 97

West-Ezier Super Blocking Buffer

W3700-050 500ml
EUR 165

West-Ezier Super Blocking Buffer

W3700-100 1L
EUR 246

PBS Blocking buffer with BSA

SB0626 1pk, 1L
EUR 83.93

TBS blocking buffer with BSA

SD8113 1pk, 1L
EUR 83.93

Permeabilization and Blocking Buffer (5X)

22017 100mL
EUR 149
Description: Minimum order quantity: 1 unit of 100mL

TrueBlack WB Blocking Buffer Kit

23013 1kit
EUR 347
Description: Minimum order quantity: 1 unit of 1kit

TrueBlack WB Blocking Buffer Kit

23013-T 1kit
EUR 125
Description: Minimum order quantity: 1 unit of 1kit

3 Blocking Buffer Sample Pack

KF17340 3 x 100 ml
EUR 205

EZBlock? T20 (TBS) Blocking Buffer

2140-1000
EUR 218

EZBlock? T20 (TBS) Blocking Buffer

2140-200
EUR 120

EZBlock? T20 (PBS) Blocking Buffer

2143-1000
EUR 218

EZBlock? T20 (PBS) Blocking Buffer

2143-200
EUR 120

ChonBlock Blocking/Sample Dilution ELISA Buffer, 100 ml

9068 100 ml
EUR 129.75
Description: ChonBlock Blocking/Sample Dilution ELISA Buffer

ELISA Plate Blocking Buffer concentrate (10X) BSA-based

80060 50 ml
EUR 202

ELISA Plate Blocking Buffer concentrate (10X) milk-based

80062 100 ml
EUR 202

Casein-based PBS Blocking Buffer Pack

AR0182 500mL/Pack
EUR 70

Casein-based TBS Blocking Buffer Pack

AR0183 500mL/Pack
EUR 70

Blocking Buffer for ICC and IHC

SF40011 10 ml
EUR 128

EZBlock? Blocking Buffer and Signal Enhancer

M4104-100
EUR 153

EZBlock? Blocking Buffer and Signal Enhancer

M4104-500
EUR 359

ELISA Binding Buffer

EBB125 125 ml
EUR 64

ELISA Binding Buffer

EBB500 500 ml
EUR 87

ELISA Binding Buffer

EBB999 1000 ml
EUR 108

Blocking Buffer for Lateral Flow (PBS based)

abx090808-500ml 500 ml
EUR 384

PBS Blocking buffer with non fat milk

SB0625 1pk, 1L
EUR 67.4

T-Pro Fast Blocking Buffer (in PBS)

JK92-W001 500ml/BT
EUR 187

T-Pro Fast Blocking Buffer (in TBS)

JK92-W002 500ml/BT
EUR 187

ELISA Plate Blocking Buffer concentrate (10X) Gelatin-based (translucent formulation)

80061 100 ml
EUR 202

ELISA Plate Blocking Buffer concentrate (10X) milk-based (translucent formulation)

80063 100 ml
EUR 202

West-Ezier Super Blocking Buffer for Phospho Antibody

W3701-010 100ml
EUR 131

West-Ezier Super Blocking Buffer for Phospho Antibody

W3701-050 500ml
EUR 188

West-Ezier Super Blocking Buffer for Phospho Antibody

W3701-100 1L
EUR 280

T-Pro Protein Free Blocking Buffer (in PBS)

JK92-W003 500ml/BT
EUR 187

T-Pro Protein Free Blocking Buffer (in TBS)

JK92-W004 500ml/BT
EUR 187

Sandwich Elisa Wash Buffer

RKB0001 20ml
EUR 119

High Performance ELISA Buffer

85R-105 55 ml
EUR 316
Description: High Performance ELISA Buffer for high sensitivity ELISA assays

10xTaq Buffer

PCRB60 4x1.5ml, 6ml
EUR 58.7

ADA buffer

AD0003 25g
EUR 64.79

Tricine buffer

20-abx082551
  • EUR 217.00
  • EUR 189.00
  • 100 g
  • 25 g

Wash Buffer

abx098952-20ml 20 ml
EUR 91

Coating Buffer

abx098970-1vial 1 vial
EUR 154

Lysis Buffer

abx098984-LysisBuffer120ml Lysis Buffer 1 (20 ml)
EUR 154

Lysis Buffer

abx098984-LysisBuffer3100ml Lysis Buffer 3 (100ml)
EUR 230

Lysis Buffer

abx098984-LysisBuffer420ml Lysis Buffer 4 (20 ml)
EUR 154

Binding Buffer

abx290019-50ml 50 ml
EUR 230

Wash Buffer

abx293002-30ml 30 ml
EUR 105

PBS Buffer

RM00012 2L
EUR 102

Fixation Buffer

22015 100mL
EUR 149
Description: Minimum order quantity: 1 unit of 100mL

Permeabilization Buffer

22016 100mL
EUR 149
Description: Minimum order quantity: 1 unit of 100mL

Binding Buffer

BB10X-50ML 50 ML
EUR 113.7

Wash Buffer

KF17356 500 ml
EUR 179

Dilution Buffer

1066-100
EUR 153

Dilution Buffer

1066-400
EUR 262

Wash Buffer

1210-200
EUR 196

RIPA Buffer

2114-100
EUR 158

RIPA Buffer

2114-500
EUR 463

West-Ez Blocking Buffer, 1 % Casein for Western Blot

W3720-050 500ml
EUR 120

West-Ez Blocking Buffer, 1 % Casein for Western Blot

W3720-100 2x500ml
EUR 147

TT Buffer (Tris-Tricine buffer) Primix powder

TD8133 1PK, 10L
EUR 76.1

10X Tris-Glycine Native Buffer (Transfer buffer)

T8052-050 500ml
EUR 80

10X Tris-Glycine Native Buffer (Transfer buffer)

T8052-100 2X500ml
EUR 104

10X Tris-Glycine Native Buffer (Transfer buffer)

T8052-101 1L
EUR 95

10X Tris-Glycine Native Buffer (Transfer buffer)

T8052-200 4X500ml
EUR 128

10X Tris-Glycine Native Buffer (Transfer buffer)

T8052-201 2X1L
EUR 128

10X Tris-Glycine Native Buffer (Transfer buffer)

T8052-401 4X1L
EUR 165

CSB; Cytokine Stabilization Buffer ELISA

CT373 5 ml
EUR 95

West-Ez Blocking Buffer, 3 % IgG Free-BSA in TBST

W3710-010 100ml
EUR 93

West-Ez Blocking Buffer, 3 % IgG Free-BSA in TBST

W3710-050 500ml
EUR 138

West-Ez Blocking Buffer, 3 % IgG Free-BSA in TBST

W3710-100 1L
EUR 182

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