elisareagents

A uniform quantitative enzyme-linked immunosorbent assay for Coxsackievirus A16 antigen in vaccine

Coxsackievirus A16 (CV-A16), considered one of main etiological brokers of hand, foot and mouth illness (HFMD), causes outbreaks of the illness in younger youngsters everywhere in the world. In an effort to promote the prevention and management of HFMD, the analysis and growth of CV-A16 vaccine have been carried out in China.
  • Nevertheless, on account of missing of a acknowledged CV-A16 antigen detection methodology, the analysis and high quality management (QC) of vaccine effectiveness are tremendously restricted. On this research, we established a quantitative enzyme-linked immunosorbent assay (Q-ELISA) to find out the antigen focus in CV-A16 vaccines that may be utilized in manufacturing in China.
  • A neutralizing antibody 16E1 was used as a seize antibody that may bind to varied CV-A16 antigens of various subgenotypes, and an antiserum from CV-A16-immunized rabbit conjugated by HRP was appropriate for detecting and quantifying CV-A16 antigens. The Q-ELISA was validated for specificity, linearity, accuracy, precision and robustness through the use of the CV-A16 antigen nationwide commonplace (NS).
  • Moreover, we utilized the Q-ELISA to quantify antigen contents of vaccine bulks from six producers and different intermediate merchandise from one producer. The outcomes indicated that the Q-ELISA can fulfill the necessities of QC for all producers concerned.
Key phrases: Coxsackievirus A16 (CV-A16); Q-ELISA; antigen; neutralizing antibody; vaccine

Consistency analysis of human serum 25-hydroxyvitamin D utilizing liquid chromatography-tandem mass spectrometry and enzyme-linked immunosorbent assay

Goal: Comparability of liquid chromatography-tandem mass spectrometry(LC-MS/MS) and enzyme-linked immunosorbent assay(ELISA) for the detection of 25(OH)D focus in human serum and the analysis of vitamin D deficiency.
Strategies: Within the serum pool of "Nationwide Institute for Vitamin and Well being, Chinese language Heart for Illness Management and Prevention", 500 serum samples of ladies aged 18 to 45 years previous had been randomly chosen, and 25(OH)D ranges had been measured by ELISA and LC-MS/MS for a similar serum pattern, respectively. The LC-MS/MS column was Waters XBridge BEH C_(18)(2. 1 mm×50 mm, 2. 5 μm).

The correlation between the 2 methodology was examined by correlation evaluation, regression evaluation and consistency check. The Endocrine Society and Institute of Drugs suggestions had been used to find out the deficiency of vitamin D, and the McNemar check, Kappa coefficient and diagnostic check had been used to diagnose the consistency of vitamin D deficiency.

Outcomes: The regression equation for the 25(OH)D focus measured by the 2 methodology was y_(LC-MS/MS)=-0. 035+1. 007×x_(ELISA)(r=0. 877), and the typical deviation between the 2 was 4. 48% and the intraclass correlation coefficient was 0. 87. The 25(OH)D focus was lower than 12 and 20 ng/mL as a criterion for vitamin D deficiency, and the Kappa coefficients had been higher than 0. 60(0. 64 and 0. 74).

Conclusion: When serum 25(OH)D stage was detected by LC-MS/MS and ELISA, the correspondence of the 2 methodology was wonderful. Taking the “gold commonplace” LC-MS/MS methodology as a reference, the ELISA methodology was used to find out human vitamin D deficiency with good sensitivity and specificity, which can be utilized for the correct and speedy detection of large-scale inhabitants samples.

Key phrases: 25(OH)D; blood check; enzyme-linked immunosorbent assay; liquid chromatography-tandem mass spectrometry.
elisareagents
elisareagents

MICROTITER PLATES POLYSTYRENE 96-WELL*F

M0187-32EA EACH
EUR 177.84

MICROTITER PLATES POLYSTYRENE 96-WELL*F

M4811-40EA EACH
EUR 148.2

MICROTITER PLATES POLYSTYRENE 96-WELL*F

M9936-40EA EACH
EUR 163.02

Micro Plate Immulon 4HBX 96well

X19735 PK50
EUR 266.76

Corning Plate 8wl Strip Eia Fb Hb Ns

2580 PK800
EUR 527.82

Adapter for 96 well plate

3510147 1unit
EUR 6697.2

Black 96 Well Plate, 1EA

X025-1EA 1EA
EUR 87.6

96 Well Semi Skirted Plate

PCR1216 PK50
EUR 150.48

96 Well Skirted PCR Plate

PCR1236 PK50
EUR 299.82

96 well PCR plate ns

AXY2164 PK50
EUR 220.02

Streptavidin coated 96-well Plate

6523-5 each
EUR 392.4

96-Well Protein Folding Plate

PFS096 kit
EUR 347.84
Description: This product includes one 96-well plate with 96 protein folding solutions, 0.5 ml in each well of the mother plate; 1.4 ml of Inclusion Body Solubilizer; 4 ml of Neutralizer. Each experiment uses 0.1 ml of the solutions from the mother plate. Each mother plate contains 0.5 ml of solutions in each well and can be used for multiple experiments of folding various proteins.

96 well filter plate (960ul each well)

SD5006 12UNIT
EUR 115.85

96 Deep Well Plate Square Well V

PCR0246 PK50
EUR 258.78

Deep Well Plate 96 Well 2.0 ML

AXYPDW20C PK50
EUR 181.26

Brand 96 Well Deep Well Plate-Stackable

BR701354-24EA PK24
EUR 144.78

UNIFILTER PLATE 96 WELL 800 UL/WELL

WHA77002803 PK25
EUR 823.08

2 x Clear 96 well Plate, 2EA

X003-2EA 2EA
EUR 78

2 x Clear 96 well Plate, 2EA

X018-2EA 2EA
EUR 78

96 WELL CLEAR PCR PLATE FOR ABI

PCR-96-AB-C 10/pk
EUR 200.4
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96-Well White Plates

M1354-2 each
EUR 157.2

96-Well Black Plates

M1355-2 each
EUR 157.2

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY

P-DW-12-HC 5/pk
EUR 182.4
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

DEEP WELL PLATE 96 WELL 2.0 ML STERIL

AXYPDW20CS PK50
EUR 216.6

Adapter for 96-well PCR plate 2 pcs.

E5825711009 PK2
EUR 150.48

INTELLI-PLATE 96-2 shallow well, low profile

MAR-102-0001-21 120 PLATES
EUR 990
Description: INTELLI-PLATE 96-2 shallow well, low profile;   2-well sitting drop, 120 plates

Clear Coated 96 Well Plate, 1EA

C107-1EA 1EA
EUR 226.8

Polystyrene-bottomed 96-well plate (ELISA)

CT361 6
EUR 87.6

96 well filter plate (no filter)

SD5006-EMPTY 12UNIT
EUR 104.89

MULTIWELL PLATE POLYSTYRENE 96-WELL FLA

M2936-100EA PK100
EUR 115.14

PCR Plate Non Skirted 96 Well

MOL2364 PK50
EUR 259.69

PCR Plate Semi Skirted 96 Well

MOL2366 PK50
EUR 251.83

96 Deep Well Plate Square Wells

PCR0240 PK100
EUR 270.18

96 Deep Well Plate Round Wells

PCR0242 PK50
EUR 232.56

96 Deep Well Plate Square WellU

PCR0256 PK50
EUR 180.12

96 Well Solid Bottom Plate Black

PCR0354 PK100
EUR 343.14

96 Well Solid Bottom Plate White

PCR0360 PK100
EUR 313.5

96 Well PCR Plate Clear Wells

PCR0972 PK50
EUR 201.78

96 Well PCR Plate Divisible In12

PCR1064 PK50
EUR 128.82

96 Well Semi-Skirted PCR Plate

PCR1106 PK50
EUR 285

S100 96-well PCR Plate Adapter

E5391070026 EACH
EUR 335.16

S200 96-well PCR Plate Adapter

E5392070038 EACH
EUR 335.16

96 Well ABI Fast Plate Pcr

PCR1200 PK50
EUR 201.78

96 Well Roche Style Qpcr Plate

PCR1210 PK50
EUR 223.44

96 Well Roche Style Pcr Plate

PCR1214 PK50
EUR 233.7

96 Well Roche Style PCR Plate

PCR1218 PK50
EUR 213.18

96 Well Skirted Plate+ Q-Stick

PCR1238 PK50
EUR 218.88

Random Access 96 Well Plate Skir

PCR1412 PK50
EUR 233.7

Nunc Lid for 96 well plate

264122 PK50
EUR 116.28

Falcon Microtest Black 96 Well Plate

353376 PK32
EUR 199.5

Benchmark Beadblaster 96 Adapter for 96 Well Plate

HOM3208 PK2
EUR 1423.86

96 WELL SITTING DROP HIGH THROUGHPUT CRYSTALLOGRAPHY PLATE.

CP-AXYGEM-96-50 10/pk
EUR 548.4
Description: Crystallography Plates; Crystallography Plates - Axygen

96 WELL CLEAR PCR HALF SKIRT AMPLIFICATION PLATE

PCR-96-HS-C 10/pk
EUR 190.8
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96 Well Half-Area Microplates

M1356-2 2 Plates
EUR 175.2

96 well DNA plate with membrane (960ul each well)

SD5007 12UNIT
EUR 291.24

96 well RNA plate with membrane (960ul each well)

SD5009 12UNIT
EUR 313.16

Benchmark Beadblaster 96 Eva Cap Mat for 96 Well Deep Well Plate

HOM3232 EACH
EUR 98.04

Wizard Classic 1 and 2 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate)

M-R-1008647 96 X 1.7 ml
EUR 316
Description: Wizard Classic 1 and 2 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate)

Wizard Cryo 1 and 2 96 Well Matrix Block Plate (96 conditions 1.7 mL volumes in a 96-Well block plate)

M-R-1008649 98 X 1.7 ml
EUR 316
Description: Wizard Cryo 1 and 2 96 Well Matrix Block Plate (96 conditions 1.7 mL volumes in a 96-Well block plate)

2 x Black Half Area 96 Well Plate, 2EA

X037-2EA 2EA
EUR 112.8

Polystyrene-bottomed 96-well plate + lid (ELISPOT)

CT365 5
EUR 93.6

96 Well Breast Tumor Genomic DNA Plate

D8235086-1 1 plate
EUR 379.2

96 Well Colon Tumor Genomic DNA Plate

D8235090-1 1 plate
EUR 379.2

96 Well Lung Tumor Genomic DNA Plate

D8235152-1 1 plate
EUR 379.2

96 Well Stomach Tumor Genomic DNA Plate

D8235248-1 1 plate
EUR 379.2

96-Well Plate PCR Products Purification Kit

BS365 5XPlates, 480prep
EUR 300.37

96-Well Plate PCR Products Purification Kit

BS3652 2xPlates, 192prep
EUR 186.19

96-Well Plate DNA Cleanup Miniprep Kit

BS369 5XPlates, 480prep
EUR 300.37

96-Well Plate DNA Cleanup Miniprep Kit

BS3692 2xPlates, 192prep
EUR 186.19

96-Well Plate Plasmid DNA Miniprep Kit

BS415 5XPlates, 480prep
EUR 300.37

96-Well Plate Plasmid DNA Miniprep Kit

BS4152 2XPlates, 192prep
EUR 186.19

96-Well Plate Viral DNA Miniprep Kit

VT92032 2XPlates, 192prep
EUR 495.86

5 x Clear 96 well Plate, 5EA

X003-5EA 5EA
EUR 96

4 x Clear 96 Well Plate, 4EA

X018-4EA 4EA
EUR 104.4

Black Half Area 96 Well Plate, 1EA

X023-1EA 1EA
EUR 96

PCR Plate 96 Well No Skirt Nat

LW2200 PK100
EUR 452.58

MULTIWELL PLATE, POLYSTYRENE 96-WELL U-B

M2186-100EA PK100
EUR 115.14

96 Deep Well Plate Round Wells U

PCR0228 PK100
EUR 166.44

96 Deep Well Plate Round Wells V

PCR0230 PK100
EUR 166.44

96 Well PCR Plate Divisible In 8

PCR1060 PK50
EUR 128.82

96 Well PCR Plate Divisible In 8

PCR1062 PK50
EUR 140.22

Analysis of human and canine Brucella canis an infection: growth and analysis of oblique enzyme-linked immunosorbent assays utilizing recombinant Brucella proteins.

Brucella canis, a Gram-negative coccobacilli belonging to the genus Brucellae, is a pathogenic bacterium that may produce infections in canines and people. A number of research have been carried out to develop diagnostic methods to detect all zoonotic Brucellae. Analysis of Brucella canis an infection is difficult as a result of lack of extremely particular and delicate diagnostic assays.

This work was divided in two phases: within the first one, had been recognized antigenic proteins in B. canis that would probably be used for serological analysis of brucellosis. Human sera optimistic for canine brucellosis an infection was used to acknowledge immunoreactive proteins that had been then recognized by performing 2D-GEL and immunoblot assays.

These spots had been analyzed utilizing MALDI TOF MS and predicted proteins had been recognized. Of the 35 protein spots analyzed, 14 proteins had been recognized and subsequently characterised utilizing bioinformatics, two of this had been chosen for the following section. Within the second section, we developed and validated an oblique enzyme-linked immunosorbent assays utilizing these recombinant proteins: inosine 5′ phosphate dehydrogenase, pyruvate dehydrogenase E1 subunit beta (PdhB) and elongation issue Tu (Tuf).

These genes had been PCR-amplified from genomic DNA of B. canis pressure Oliveri, cloned, and expressed in Escherichia coli. Recombinant proteins had been purified by metallic affinity chromatography, and used as antigens in oblique ELISA. Serum samples from wholesome and B. canis-infected people and canines had been used to guage the efficiency of oblique ELISAs. Our outcomes counsel that PdhB and Tuf proteins could possibly be used as antigens for serologic detection of B. canis an infection in people, however not in canines.

The usage of recombinant antigens in iELISA assays to detect B. canis-specific antibodies in human serum could possibly be a beneficial instrument to enhance analysis of human brucellosis attributable to B. canis.

Key phrases: Micro organism; Bacteriology; Organic sciences; Brucella canis str. Oliveri; Brucellosis; ELISA check; Well being sciences; Immunoproteome: people; Infectious illness; Microbiology; Microorganism; Proteins; Proteomics; Veterinary drugs; Zoonoses.

EZBlock? (PBS) Blocking Buffer

2128-1000 each
EUR 274.8

EZBlock? (PBS) Blocking Buffer

2128-200 each
EUR 164.4

Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed

ELISA-1 1
EUR 242.4

TrueBlack WB Blocking Buffer Kit

23013 1kit
EUR 416.4
Description: Minimum order quantity: 1 unit of 1kit

TrueBlack WB Blocking Buffer Kit

23013-T 1kit
EUR 150
Description: Minimum order quantity: 1 unit of 1kit

Blocking Buffer and Coating Stabilizer

abx090811-1000ml 1000 ml
EUR 627.6

3 Blocking Buffer Sample Pack

KF17340 3 x 100 ml
EUR 246

10 x Universal Blocking Buffer

GR103005 200 mL
EUR 154.8

PBS Blocking buffer with BSA

SB0626 1pk, 1L
EUR 100.72

TBS blocking buffer with BSA

SD8113 1pk, 1L
EUR 100.72

West-Ezier Super Blocking Buffer

W3700-010 100ml
EUR 116.4

West-Ezier Super Blocking Buffer

W3700-050 500ml
EUR 198

West-Ezier Super Blocking Buffer

W3700-100 1L
EUR 295.2

EZBlock? T20 (TBS) Blocking Buffer

2140-1000 each
EUR 261.6

EZBlock? T20 (TBS) Blocking Buffer

2140-200 each
EUR 144

EZBlock? T20 (PBS) Blocking Buffer

2143-1000 each
EUR 261.6

EZBlock? T20 (PBS) Blocking Buffer

2143-200 each
EUR 144

ChonBlock Blocking/Sample Dilution ELISA Buffer, 100 ml

9068 100 ml
EUR 155.7
Description: ChonBlock Blocking/Sample Dilution ELISA Buffer

ELISA Plate Blocking Buffer concentrate (10X) BSA-based

80060 50 ml
EUR 242.4

Casein-based PBS Blocking Buffer Pack

AR0182 500mL/Pack
EUR 84

Casein-based TBS Blocking Buffer Pack

AR0183 500mL/Pack
EUR 84

Blocking Buffer for ICC and IHC

SF40011 10 ml
EUR 153.6

Corning 3D Clear Blocking Buffer - 30mL

5736 EACH
EUR 108.3

Corning 3D Clear Blocking Buffer - 100mL

5737 EACH
EUR 294.12

EZBlock? Blocking Buffer and Signal Enhancer

M4104-100 each
EUR 183.6

EZBlock? Blocking Buffer and Signal Enhancer

M4104-500 each
EUR 430.8

Corning 3D Clear Starter Kit - Includes Reagent/Antibody Buffer/Blocking Buffer/Penetration Buffer/Washing Buffers

5730 EACH
EUR 864.12

Blocking Buffer for Lateral Flow (PBS based)

abx090808-500ml 500 ml
EUR 460.8

T-Pro Fast Blocking Buffer (in PBS)

JK92-W001 500ml/BT
EUR 224.4

T-Pro Fast Blocking Buffer (in TBS)

JK92-W002 500ml/BT
EUR 224.4

PBS Blocking buffer with non fat milk

SB0625 1pk, 1L
EUR 80.88

Blocking Buffer 4 (Multiple Epigenetics Assay Kits)

52100 50 ml
EUR 95
Description: This Blocking Buffer 4 is optimized for minimizing background in various chemiluminescent methyltransferase, demethylase, and TET assays.

Blocking Buffer 1 (Various Epigenetics Assay Kits)

79556 50 ml
EUR 100
Description: This Blocking Buffer 1 is optimized for minimizing background in various BPS Bioscience assay kits. The BPS Bioscience Blocking Buffer improves S/B ratio of HMTs NSD2 and SetDB2 ELISAs with respect to competitor blocking buffer by both decreasing background (0% Activity control, no enzyme) and increasing assay signal (100% Activity control, enzyme without inhibitor).

Blocking Buffer 2 (BPS Immunotherapy Assay Kits)

79728 50 ml
EUR 110

Blocking Buffer 3 (Multiple PARP Assay Kits)

79743 25 ml x 2
EUR 105
Description: This Blocking Buffer 3 is optimized for minimizing background in multiple PARP BPS Bioscience Assay Kits. _x000D__x000D_

ELISA Binding Buffer

EBB125 125 ml
EUR 76.8

ELISA Binding Buffer

EBB500 500 ml
EUR 104.4

ELISA Binding Buffer

EBB999 1000 ml
EUR 129.6

T-Pro Protein Free Blocking Buffer (in PBS)

JK92-W003 500ml/BT
EUR 224.4

T-Pro Protein Free Blocking Buffer (in TBS)

JK92-W004 500ml/BT
EUR 224.4

West-Ezier Super Blocking Buffer for Phospho Antibody

W3701-010 100ml
EUR 157.2

West-Ezier Super Blocking Buffer for Phospho Antibody

W3701-050 500ml
EUR 225.6

West-Ezier Super Blocking Buffer for Phospho Antibody

W3701-100 1L
EUR 336

High Performance ELISA Buffer

85R-105 55 ml
EUR 379.2
Description: High Performance ELISA Buffer for high sensitivity ELISA assays

ELISA Assay Buffer, 20x

GR103001 1 L
EUR 106.8

ELISA Assay Buffer, 80x

GR103002 1 L
EUR 190.8

EIA Buffer for ELISA

GR103069 100 mL
EUR 82.8

Sandwich Elisa Wash Buffer

RKB0001 20ml
EUR 142.8

West-Ez Blocking Buffer, 1 % Casein for Western Blot

W3720-050 500ml
EUR 144

West-Ez Blocking Buffer, 1 % Casein for Western Blot

W3720-100 2x500ml
EUR 176.4

ADA buffer

AD0003 25g
EUR 77.75

Wash Buffer

abx098952-20ml 20 ml
EUR 109.2

Coating Buffer

abx098970-1vial 1 vial
EUR 184.8

Lysis Buffer

abx098984-LysisBuffer120ml Lysis Buffer 1 (20 ml)
EUR 184.8

Lysis Buffer

abx098984-LysisBuffer3100ml Lysis Buffer 3 (100ml)
EUR 276

Lysis Buffer

abx098984-LysisBuffer420ml Lysis Buffer 4 (20 ml)
EUR 184.8

Tricine buffer

20-abx082551
  • EUR 260.40
  • EUR 226.80
  • 100 g
  • 25 g

Binding Buffer

abx290019-50ml 50 ml
EUR 276

Wash Buffer

abx293002-30ml 30 ml
EUR 126

Binding Buffer

BB10X-50ML 50 ML
EUR 136.44

Wash Buffer

KF17356 500 ml
EUR 214.8

RIPA Buffer

GR103019 50 mL
EUR 70.8

Stripping Buffer

GR103020 500 mL
EUR 106.8

Anode Buffer

GR103036 1 L
EUR 106.8

Cathode Buffer

GR103037 1 L
EUR 130.8

10xTaq Buffer

PCRB60 4x1.5ml, 6ml
EUR 70.44

PBS Buffer

RM00012 2L
EUR 122.4

Phosphate Buffer

PB1 1L
EUR 94.62

Dilution Buffer

1066-100 each
EUR 183.6

Dilution Buffer

1066-400 each
EUR 314.4

Wash Buffer

1210-200 each
EUR 235.2

RIPA Buffer

2114-100 each
EUR 189.6

RIPA Buffer

2114-500 each
EUR 555.6

CSB; Cytokine Stabilization Buffer ELISA

CT373 5 ml
EUR 114

Sorry! No related Post.

Leave a Reply

Your email address will not be published.