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A uniform quantitative enzyme-linked immunosorbent assay for Coxsackievirus A16 antigen in vaccine

Coxsackievirus A16 (CV-A16), considered one of main etiological brokers of hand, foot and mouth illness (HFMD), causes outbreaks of the illness in younger youngsters everywhere in the world. In an effort to promote the prevention and management of HFMD, the analysis and growth of CV-A16 vaccine have been carried out in China.
  • Nevertheless, on account of missing of a acknowledged CV-A16 antigen detection methodology, the analysis and high quality management (QC) of vaccine effectiveness are tremendously restricted. On this research, we established a quantitative enzyme-linked immunosorbent assay (Q-ELISA) to find out the antigen focus in CV-A16 vaccines that may be utilized in manufacturing in China.
  • A neutralizing antibody 16E1 was used as a seize antibody that may bind to varied CV-A16 antigens of various subgenotypes, and an antiserum from CV-A16-immunized rabbit conjugated by HRP was appropriate for detecting and quantifying CV-A16 antigens. The Q-ELISA was validated for specificity, linearity, accuracy, precision and robustness through the use of the CV-A16 antigen nationwide commonplace (NS).
  • Moreover, we utilized the Q-ELISA to quantify antigen contents of vaccine bulks from six producers and different intermediate merchandise from one producer. The outcomes indicated that the Q-ELISA can fulfill the necessities of QC for all producers concerned.
Key phrases: Coxsackievirus A16 (CV-A16); Q-ELISA; antigen; neutralizing antibody; vaccine

Consistency analysis of human serum 25-hydroxyvitamin D utilizing liquid chromatography-tandem mass spectrometry and enzyme-linked immunosorbent assay

Goal: Comparability of liquid chromatography-tandem mass spectrometry(LC-MS/MS) and enzyme-linked immunosorbent assay(ELISA) for the detection of 25(OH)D focus in human serum and the analysis of vitamin D deficiency.
Strategies: Within the serum pool of "Nationwide Institute for Vitamin and Well being, Chinese language Heart for Illness Management and Prevention", 500 serum samples of ladies aged 18 to 45 years previous had been randomly chosen, and 25(OH)D ranges had been measured by ELISA and LC-MS/MS for a similar serum pattern, respectively. The LC-MS/MS column was Waters XBridge BEH C_(18)(2. 1 mm×50 mm, 2. 5 μm).

The correlation between the 2 methodology was examined by correlation evaluation, regression evaluation and consistency check. The Endocrine Society and Institute of Drugs suggestions had been used to find out the deficiency of vitamin D, and the McNemar check, Kappa coefficient and diagnostic check had been used to diagnose the consistency of vitamin D deficiency.

Outcomes: The regression equation for the 25(OH)D focus measured by the 2 methodology was y_(LC-MS/MS)=-0. 035+1. 007×x_(ELISA)(r=0. 877), and the typical deviation between the 2 was 4. 48% and the intraclass correlation coefficient was 0. 87. The 25(OH)D focus was lower than 12 and 20 ng/mL as a criterion for vitamin D deficiency, and the Kappa coefficients had been higher than 0. 60(0. 64 and 0. 74).

Conclusion: When serum 25(OH)D stage was detected by LC-MS/MS and ELISA, the correspondence of the 2 methodology was wonderful. Taking the “gold commonplace” LC-MS/MS methodology as a reference, the ELISA methodology was used to find out human vitamin D deficiency with good sensitivity and specificity, which can be utilized for the correct and speedy detection of large-scale inhabitants samples.

Key phrases: 25(OH)D; blood check; enzyme-linked immunosorbent assay; liquid chromatography-tandem mass spectrometry.
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elisareagents

Plate 96 Well Immulon II - PK50

3455 PK50
EUR 318.6

MICROTITER PLATES POLYSTYRENE 96-WELL*F - EACH

M0187-32EA EACH
EUR 229.13

Plate 96 Well Immulon 1 FB - PK50

N3355 PK50
EUR 233.55

Micro Plate Immulon 4HBX 96well - PK50

X19735 PK50
EUR 348.3

Standard Microtiter Plate Carrier

MAAB-0827 each
EUR 386.95

IKA Microtiter/Deep-Well Plate Insert for Matrix Delta Plus - EACH

SHA5044 EACH
EUR 811.35

Microtiter Plates (P96FL)

P96FL 96Well
EUR 12

Microtiter Plates (P96OD)

P96OD 96Well
EUR 12

Microtiter Plates (P96UV)

P96UV 96Well
EUR 27

Microtiter Plates (P96WT)

P96WT 96Well
EUR 12

Microtiter Plates (P96BCC)

P96BCC 96Well
EUR 22

Microtiter Plates (P96CCC)

P96CCC 96Well
EUR 22

Microtiter plate buckets for swing-bucket-rotor A-4-62-MTP - PK4

E5810702003 PK4
EUR 1287.9

IKA Matrix Orbital Delta FP with Universal Holder for Microtiter Plates - EACH

SHA5022 EACH
EUR 3109.05

Falcon Microtest Black 96 Well Plate - PK32

353376 PK32
EUR 244.35

Corning Microtiter Hotel for 4x Microplates for Shaking Incubator 6791 - EACH

480147 EACH
EUR 280.8

96 Well Impact MicroBatch Plate, 80/CSE

M-673101 80 PLATES
EUR 808
Description: 96 Well Impact MicroBatch Plate, 80/CSE

96-Well Plate

1996-2 each
EUR 124.8

Microbiol blue skirted PCR plate 96 well 10pcs - EACH

E0030129318 EACH
EUR 103.95

Microbiol clear skirted PCR plate 96 well 10pcs - EACH

E0030129300 EACH
EUR 103.95

96-well Array Plate

AP-001C 1 plate
EUR 232
Description: Chromatography Reagents

96-Well Filter Plate

FCM-B01M 1plate
EUR 21.4
Description: The membrane of this plate has a consistent physical structure with a smooth surface morphology, this makes it ideal for use in bead based assays as the microspheres do not get trapped in the membrane, allowing for efficient bead recovery. The filter plate is suggested no more than 350μL and no less than 1.2μm loading.

96 well filter plate (960ul each well)

SD5006 12UNIT
EUR 115.85

Microbiol semi skirted blue PCR plate 96 well 10pcs - EACH

E0030129334 EACH
EUR 103.95

Block for one Deep Well Micro Plate

H5000-DWMP 1 PC
EUR 494.19

Black 96 Well Plate, 1EA

X025-1EA 1EA
EUR 19

NanoECM®; 96 Well Plate

9601 each
EUR 38.7

Adapter for 96 well plate

3510147 1unit
EUR 4422

Microbiol clear semi skirted clear PCR plate 96 well 10pcs - EACH

E0030129326 EACH
EUR 103.95

UNIFILTER PLATE 96 WELL 800 UL/WELL - PK25

WHA77002803 PK25
EUR 1101.6

Cell Culture Plate, 96 Well

CCP01096-100Case 100 / Case
EUR 154.24

384-Well Microarray Plate V-wells (50)

AB-1056 each
EUR 322.1

96 WELL PCR SEGMENTED PLATE

PCR-96-SG-C 10/pk
EUR 174
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

Deep Well Plate 96 Well 2.0 ML - PK50

AXYPDW20C PK50
EUR 221.4

NanoAligned®; 96 Well Plate

9602 each
EUR 41.4

Tissue Culture Plate, 96 Well

TCP20-96 1 UNIT
EUR 64.18

2 x Clear 96 well Plate, 2EA

X003-2EA 2EA
EUR 13

2 x Clear 96 well Plate, 2EA

X018-2EA 2EA
EUR 13

96 Deep Well Plate Square Well V - PK50

PCR0246 PK50
EUR 363.15

384-Well Microarray Plate cylindrical wells (50)

AB-1055 each
EUR 316.1

96-Well Protein Folding Plate

PFS096 kit
EUR 347.84
Description: This product includes one 96-well plate with 96 protein folding solutions, 0.5 ml in each well of the mother plate; 1.4 ml of Inclusion Body Solubilizer; 4 ml of Neutralizer. Each experiment uses 0.1 ml of the solutions from the mother plate. Each mother plate contains 0.5 ml of solutions in each well and can be used for multiple experiments of folding various proteins.

Aligned Nanofibers 96 Well Plate

3D00012-96 700 nm-PCLs
EUR 132

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY

P-DW-12-HC 5/pk
EUR 182.4
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

Block for one Deep Well Micro Plate - EACH

MIX7222 EACH
EUR 714.15

96-well Black Polystyrene Plate

MBS258058-1Plate 1Plate
EUR 115

96-well Black Polystyrene Plate

MBS258058-5x1Plate 5x1Plate
EUR 340

Streptavidin coated 96-well Plate

6523-5 each
EUR 392.4

384 WELL BLACK RIGI PLATE PCR MICROPLATE

PCR-384-RGD-BK 20/pk
EUR 585.6
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

Wizard Cryo 1 and 2 96 Well Matrix Block Plate (96 conditions 1.7 mL volumes in a 96-Well block plate)

M-R-1008649 98 X 1.7 ml
EUR 316
Description: Wizard Cryo 1 and 2 96 Well Matrix Block Plate (96 conditions 1.7 mL volumes in a 96-Well block plate)

Brand 96 Well Deep Well Plate-Stackable - PK24

BR701354-24EA PK24
EUR 183.6

Microbiol blue PCR plate 384 well 10pcs - EACH

E0030129350 EACH
EUR 153.9

96 well PCR plate ns - PK50

AXY2164 PK50
EUR 268.65

DEEP WELL PLATE 96 WELL 2.0 ML STERIL - PK50

AXYPDW20CS PK50
EUR 270

96 Well Roche Style PCR Plate

PCR1218 PK50
EUR 223.74

Clear Coated 96 Well Plate, 1EA

C107-1EA 1EA
EUR 162

96 Well Skirted PCR Plate - PK50

PCR1236 PK50
EUR 433.35

96 well filter plate (no filter)

SD5006-EMPTY 12UNIT
EUR 104.89

96 Well Semi Skirted Plate - PK50

PCR1216 PK50
EUR 229.5

96 well DNA plate with membrane (960ul each well)

SD5007 12UNIT
EUR 291.24

96 well RNA plate with membrane (960ul each well)

SD5009 12UNIT
EUR 313.16

Adapter for 96 well plate, pack of 2

IPD9600-96 each
EUR 1337.97

96 Well PCR Plate Clear Wells - PK50

PCR0972 PK50
EUR 290.25

BioMag® 96-Well Plate Separator

8MB4109S-1 1magnet
EUR 955

96 Deep Well Plate Round Wells - PK50

PCR0242 PK50
EUR 329.4

384 WELL CLEAR RIGI PLATE PCR MICROPLATE. CLEAR

PCR-384-RGD-C 20/pk
EUR 550.8
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96 Deep Well Plate Square WellU - PK50

PCR0256 PK50
EUR 261.9

96 Deep Well Plate Square Wells - PK100

PCR0240 PK100
EUR 378

96 WELL BARCODED H1-H12, CLEAR PCR FULL SKIRT PCR AMPLIFICATION MICRO PLATE

PCR-96-FS-C-PBC 10/pk
EUR 201.6
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

INTELLI-PLATE 96-2 Shallow well;  2-well sitting drop, 120 plates

MAR-102-0001-20 120 PLATES
EUR 990
Description: INTELLI-PLATE 96-2 Shallow well;  2-well sitting drop, 120 plates

Wizard Classic 1 and 2 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate)

M-R-1008647 96 X 1.7 ml
EUR 316
Description: Wizard Classic 1 and 2 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate)

VersiPlate, 96-well PCR Plate - 25 plates

AB-1800 each
EUR 149.88

5 x Clear 96 well Plate, 5EA

X003-5EA 5EA
EUR 25

4 x Clear 96 Well Plate, 4EA

X018-4EA 4EA
EUR 31

Polystyrene-bottomed 96-well plate (ELISA)

CT361 6
EUR 87.6

Nunc Lid for 96 well plate - PK50

264122 PK50
EUR 143.1

96 WELL CLEAR PCR PLATE FOR ABI

PCR-96-AB-C 10/pk
EUR 200.4
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96 Well ABI Fast Plate Pcr - PK50

PCR1200 PK50
EUR 290.25

96 Well ExoELISA plate (12x8 well strips, pack of 10 plates)

EXOEL-96P-1 10 Plates
EUR 255.6

Strip plates 96-well 12xF8 non dividable immunoGrade HB - PK100

F782305 PK100
EUR 722.25

Black Half Area 96 Well Plate, 1EA

X023-1EA 1EA
EUR 25

96 Well PCR Plate Divisible In 8

PCR1062 PK50
EUR 154.44

Analysis of human and canine Brucella canis an infection: growth and analysis of oblique enzyme-linked immunosorbent assays utilizing recombinant Brucella proteins.

Brucella canis, a Gram-negative coccobacilli belonging to the genus Brucellae, is a pathogenic bacterium that may produce infections in canines and people. A number of research have been carried out to develop diagnostic methods to detect all zoonotic Brucellae. Analysis of Brucella canis an infection is difficult as a result of lack of extremely particular and delicate diagnostic assays.

This work was divided in two phases: within the first one, had been recognized antigenic proteins in B. canis that would probably be used for serological analysis of brucellosis. Human sera optimistic for canine brucellosis an infection was used to acknowledge immunoreactive proteins that had been then recognized by performing 2D-GEL and immunoblot assays.

These spots had been analyzed utilizing MALDI TOF MS and predicted proteins had been recognized. Of the 35 protein spots analyzed, 14 proteins had been recognized and subsequently characterised utilizing bioinformatics, two of this had been chosen for the following section. Within the second section, we developed and validated an oblique enzyme-linked immunosorbent assays utilizing these recombinant proteins: inosine 5′ phosphate dehydrogenase, pyruvate dehydrogenase E1 subunit beta (PdhB) and elongation issue Tu (Tuf).

These genes had been PCR-amplified from genomic DNA of B. canis pressure Oliveri, cloned, and expressed in Escherichia coli. Recombinant proteins had been purified by metallic affinity chromatography, and used as antigens in oblique ELISA. Serum samples from wholesome and B. canis-infected people and canines had been used to guage the efficiency of oblique ELISAs. Our outcomes counsel that PdhB and Tuf proteins could possibly be used as antigens for serologic detection of B. canis an infection in people, however not in canines.

The usage of recombinant antigens in iELISA assays to detect B. canis-specific antibodies in human serum could possibly be a beneficial instrument to enhance analysis of human brucellosis attributable to B. canis.

Key phrases: Micro organism; Bacteriology; Organic sciences; Brucella canis str. Oliveri; Brucellosis; ELISA check; Well being sciences; Immunoproteome: people; Infectious illness; Microbiology; Microorganism; Proteins; Proteomics; Veterinary drugs; Zoonoses.

ELISA SYNBLOCK

MBS238287-5x500mL 5x500mL
EUR 1325

ELISA SYNBLOCK

MBS238288-1L 1L
EUR 520

ELISA SYNBLOCK

MBS238288-5x1L 5x1L
EUR 2210

ELISA SYNBLOCK Reagent

GWB-Q00224 100 ml Ask for price

ELISA SYNBLOCK Reagent

GWB-Q00225 500 ml Ask for price

ELISA SYNBLOCK Reagent

GWB-Q00226 1000 ml Ask for price

OOSA09557-1L - ELISA SYNBLOCK Accessory Reagent

OOSA09557-1L 1000ml
EUR 519

OOSA09555-100ML - ELISA SYNBLOCK Accessory Reagent

OOSA09555-100ML 100ml
EUR 129

OOSA09556-500ML - ELISA SYNBLOCK Accessory Reagent

OOSA09556-500ML 500ml
EUR 299

Blocking Buffer for ELISA

abx090807-500ml 500 ml
EUR 460.8

Blocking Buffer for ELISA

GWB-E120C6 500 ml Ask for price

Blocking Buffer for ELISA

MBS318404-500mL 500mL
EUR 335

Blocking Buffer for ELISA

MBS318404-5x500mL 5x500mL
EUR 1315

ELISA Plate Blocking Buffer

E-ELIR-003-24Tests 24 Tests Ask for price

ELISA Plate Blocking Buffer

E-ELIR-003-48Tests 48 Tests
EUR 110

ELISA Plate Blocking Buffer

E-ELIR-003-96Tests 96 Tests
EUR 35

ELISA Plate Blocking Buffer

E-ELIR-003-96Tests10 96 Tests *10
EUR 350

ELISA Plate Blocking Buffer

E-ELIR-003-96Tests5 96 Tests *5
EUR 175

ELISA Plate Blocking Buffer

MBS2571969-100mL 100mL
EUR 110

ELISA Plate Blocking Buffer

MBS2571969-1L 1L
EUR 165

PanBlock ELISA Blocking Buffer

BUF81201-100 each
EUR 81.12
Description: Blocking Buffer for antigen-down and sandwich ELISA. PanBlock is for antigen-down and sandwich ELISAs and for those with high background problems or cross-react with mammalian buffers.

PanBlock ELISA Blocking Buffer

BUF81201-1000 each
EUR 384.98
Description: Blocking Buffer for antigen-down and sandwich ELISA. PanBlock is for antigen-down and sandwich ELISAs and for those with high background problems or cross-react with mammalian buffers.

Synthetic Blocking Buffer-for ELISA

4520A 1 L
EUR 157.3

Synthetic Blocking Buffer-for ELISA

4520L 500 ML
EUR 116.6

Neptune Block ELISA Blocking Buffer

MBS258384-100mL 100mL
EUR 160

Neptune Block ELISA Blocking Buffer

MBS258384-1L 1L
EUR 380

Neptune Block ELISA Blocking Buffer

MBS258384-500mL 500mL
EUR 260

Neptune Block ELISA Blocking Buffer

MBS258384-5x1L 5x1L
EUR 1595

Alternative Block ELISA Blocking Buffer, Synthetic

MBS258395-100mL 100mL
EUR 160

Alternative Block ELISA Blocking Buffer, Synthetic

MBS258395-1L 1L
EUR 345

Alternative Block ELISA Blocking Buffer, Synthetic

MBS258395-500mL 500mL
EUR 230

Alternative Block ELISA Blocking Buffer, Synthetic

MBS258395-5x1L 5x1L
EUR 1435

Blocking Buffer

abx098972-1vial 1 vial
EUR 184.8

Blocking Buffer

ML044-100ML 1 unit
EUR 4.75
Description: Blocking Buffer

Blocking Buffer

ML044-500ML 1 unit
EUR 16.62
Description: Blocking Buffer

Blocking Buffer

RM01757 20mL
EUR 280

Blocking Buffer

abx098972-100l 100 µl
EUR 93.75

Blocking Buffer

abx098972-1ml 1 ml Ask for price

Blocking Buffer

abx098972-200l 200 µl Ask for price

Alternative Block™ ELISA Blocking Buffer, Synthetic

6299 100 mL
EUR 52.25

Alternative Block™ ELISA Blocking Buffer, Synthetic

6300 500 mL
EUR 145

Alternative Block™ ELISA Blocking Buffer, Synthetic

6301 1 L
EUR 290

5% Blocking Buffer

E-IR-R107-10mL 10mL
EUR 10

5% Blocking Buffer

E-IR-R107-50mL 50mL
EUR 30

5% Blocking Buffer

E-IR-R107-5mL 5mL
EUR 8

5% Blocking Buffer

E-IR-R107-each each Ask for price

OOMA00026-500ML - Blocking Buffer for ELISA

OOMA00026-500ML 500ML
EUR 349

Western Blocking Buffer

EZWB04-4-100mL 100mL
EUR 10.8

Western Blocking Buffer

EZWB04-4-500mL 500mL
EUR 38.4

Western Blocking Buffer

MBS355690-100mL 100mL
EUR 180

Western Blocking Buffer

MBS355690-5x100mL 5x100mL
EUR 500

Monster Block ELISA Blocking Buffer, Non-Mammalian

MBS258394-100mL 100mL
EUR 160

Monster Block ELISA Blocking Buffer, Non-Mammalian

MBS258394-1L 1L
EUR 345

Monster Block ELISA Blocking Buffer, Non-Mammalian

MBS258394-500mL 500mL
EUR 230

Monster Block ELISA Blocking Buffer, Non-Mammalian

MBS258394-5x1L 5x1L
EUR 1435

Blocking Buffer for WB

GWB-41822F 500 ml Ask for price

5% BSA Blocking Buffer

MBS2567133-10mL 10mL
EUR 80

5% BSA Blocking Buffer

MBS2567133-50mL 50mL
EUR 95

5% BSA Blocking Buffer

MBS2567133-5mL 5mL
EUR 80

5% BSA Blocking Buffer

MBS2567133-5x50mL 5x50mL
EUR 390

T-Pro Blocking Buffer

JK92-W005 500ml/BT
EUR 60

TBS Blocking Buffer Pack

AR0143 200mL/pack
EUR 78

Blocking Buffer, 10X, 250ML

X109-250ML 250ML
EUR 360

Blocking Buffer, 10X, 25ML

X109-25ML 25ML
EUR 59

ELISA Plate Blocking Buffer concentrate (10X) BSA-based

80060 50 ml
EUR 242.4

Monster Block™ ELISA Blocking Buffer, Non-Mammalian

6295 100 mL
EUR 52.25

Monster Block™ ELISA Blocking Buffer, Non-Mammalian

6296 500 mL
EUR 145

Monster Block™ ELISA Blocking Buffer, Non-Mammalian

6297 1 L
EUR 290

General Blocker Blocking Buffer

640 1 L
EUR 260.5

General Blocker Blocking Buffer

MBS258397-100mL 100mL
EUR 150

General Blocker Blocking Buffer

MBS258397-1L 1L
EUR 320

General Blocker Blocking Buffer

MBS258397-500mL 500mL
EUR 230

General Blocker Blocking Buffer

MBS258397-5x1L 5x1L
EUR 1325

EZBlock? (TBS) Blocking Buffer

2117-1000 each
EUR 254.4
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