elisareagents

A uniform quantitative enzyme-linked immunosorbent assay for Coxsackievirus A16 antigen in vaccine

Coxsackievirus A16 (CV-A16), considered one of main etiological brokers of hand, foot and mouth illness (HFMD), causes outbreaks of the illness in younger youngsters everywhere in the world. In an effort to promote the prevention and management of HFMD, the analysis and growth of CV-A16 vaccine have been carried out in China.
  • Nevertheless, on account of missing of a acknowledged CV-A16 antigen detection methodology, the analysis and high quality management (QC) of vaccine effectiveness are tremendously restricted. On this research, we established a quantitative enzyme-linked immunosorbent assay (Q-ELISA) to find out the antigen focus in CV-A16 vaccines that may be utilized in manufacturing in China.
  • A neutralizing antibody 16E1 was used as a seize antibody that may bind to varied CV-A16 antigens of various subgenotypes, and an antiserum from CV-A16-immunized rabbit conjugated by HRP was appropriate for detecting and quantifying CV-A16 antigens. The Q-ELISA was validated for specificity, linearity, accuracy, precision and robustness through the use of the CV-A16 antigen nationwide commonplace (NS).
  • Moreover, we utilized the Q-ELISA to quantify antigen contents of vaccine bulks from six producers and different intermediate merchandise from one producer. The outcomes indicated that the Q-ELISA can fulfill the necessities of QC for all producers concerned.
Key phrases: Coxsackievirus A16 (CV-A16); Q-ELISA; antigen; neutralizing antibody; vaccine

Consistency analysis of human serum 25-hydroxyvitamin D utilizing liquid chromatography-tandem mass spectrometry and enzyme-linked immunosorbent assay

Goal: Comparability of liquid chromatography-tandem mass spectrometry(LC-MS/MS) and enzyme-linked immunosorbent assay(ELISA) for the detection of 25(OH)D focus in human serum and the analysis of vitamin D deficiency.
Strategies: Within the serum pool of "Nationwide Institute for Vitamin and Well being, Chinese language Heart for Illness Management and Prevention", 500 serum samples of ladies aged 18 to 45 years previous had been randomly chosen, and 25(OH)D ranges had been measured by ELISA and LC-MS/MS for a similar serum pattern, respectively. The LC-MS/MS column was Waters XBridge BEH C_(18)(2. 1 mm×50 mm, 2. 5 μm).

The correlation between the 2 methodology was examined by correlation evaluation, regression evaluation and consistency check. The Endocrine Society and Institute of Drugs suggestions had been used to find out the deficiency of vitamin D, and the McNemar check, Kappa coefficient and diagnostic check had been used to diagnose the consistency of vitamin D deficiency.

Outcomes: The regression equation for the 25(OH)D focus measured by the 2 methodology was y_(LC-MS/MS)=-0. 035+1. 007×x_(ELISA)(r=0. 877), and the typical deviation between the 2 was 4. 48% and the intraclass correlation coefficient was 0. 87. The 25(OH)D focus was lower than 12 and 20 ng/mL as a criterion for vitamin D deficiency, and the Kappa coefficients had been higher than 0. 60(0. 64 and 0. 74).

Conclusion: When serum 25(OH)D stage was detected by LC-MS/MS and ELISA, the correspondence of the 2 methodology was wonderful. Taking the “gold commonplace” LC-MS/MS methodology as a reference, the ELISA methodology was used to find out human vitamin D deficiency with good sensitivity and specificity, which can be utilized for the correct and speedy detection of large-scale inhabitants samples.

Key phrases: 25(OH)D; blood check; enzyme-linked immunosorbent assay; liquid chromatography-tandem mass spectrometry.
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elisareagents

Micro Plate Immulon 4HBX 96well

X19735 PK50
EUR 309.6

IKA Microtiter/Deep-Well Plate Insert for Matrix Delta Plus

SHA5044 EACH
EUR 721.2

Microtiter Plates (P96FL)

P96FL 96Well
EUR 10
Description: Black Flat-Bottom 96-Well Plate, Nonsterile, with Lid, for Fluorometric Assays.

Microtiter Plates (P96OD)

P96OD 96Well
EUR 9
Description: Clear Flat-Bottom 96-Well Plate, Nonsterile, with Lid, for OD Assays at >350nm.

Microtiter Plates (P96UV)

P96UV 96Well
EUR 23
Description: Clear Flat-Bottom 96-Well UV Plate, Nonsterile, with Lid, for OD Assays at >230nm.

Microtiter Plates (P96WT)

P96WT 96Well
EUR 9
Description: White Opaque Flat-Bottom 96-Well Plate, Nonsterile, with Lid, for Luminescence Assays.

Microtiter Plates (P96BCC)

P96BCC 96Well
EUR 19
Description: Black Clear Bottom 96-Well Plate, Sterile, with Lid, for Cell-based Fluorometric Assays.

Microtiter Plates (P96CCC)

P96CCC 96Well
EUR 19
Description: Clear Flat-Bottom 96-Well Plate, Sterile, with Lid, for Cell-based Colorimetric Assays.

Microtiter plate buckets for swing-bucket-rotor A-4-62-MTP

E5810702003 PK4
EUR 1144.8

IKA Matrix Orbital Delta FP with Universal Holder for Microtiter Plates

SHA5022 EACH
EUR 2763.6

Falcon Microtest Black 96 Well Plate

353376 PK32
EUR 217.2

Corning Microtiter Hotel for 4x Microplates for Shaking Incubator 6791

480147 EACH
EUR 249.6

96 Well Impact MicroBatch Plate, 80/CSE

M-673101 80 PLATES
EUR 808
Description: 96 Well Impact MicroBatch Plate, 80/CSE

Microbiol blue skirted PCR plate 96 well 10pcs

E0030129318 EACH
EUR 92.4

96-Well Plate

1996-2 each
EUR 124.8

Microbiol clear skirted PCR plate 96 well 10pcs

E0030129300 EACH
EUR 92.4

Microbiol semi skirted blue PCR plate 96 well 10pcs

E0030129334 EACH
EUR 92.4

96-Well Filter Plate

FCM-B01M 1plate
EUR 21.4
Description: The membrane of this plate has a consistent physical structure with a smooth surface morphology, this makes it ideal for use in bead based assays as the microspheres do not get trapped in the membrane, allowing for efficient bead recovery. The filter plate is suggested no more than 350μL and no less than 1.2μm loading.

96 well filter plate (960ul each well)

SD5006 12UNIT
EUR 115.85

Microbiol clear semi skirted clear PCR plate 96 well 10pcs

E0030129326 EACH
EUR 92.4

UNIFILTER PLATE 96 WELL 800 UL/WELL

WHA77002803 PK25
EUR 979.2

Deep Well Plate 96 Well 2.0 ML

AXYPDW20C PK50
EUR 196.8

Block for one Deep Well Micro Plate

MIX7222 EACH
EUR 634.8

Block for one Deep Well Micro Plate

H5000-DWMP 1 PC
EUR 479.8

96 Deep Well Plate Square Well V

PCR0246 PK50
EUR 322.8

Microbiol blue PCR plate 384 well 10pcs

E0030129350 EACH
EUR 136.8

96 well PCR plate ns

AXY2164 PK50
EUR 238.8

Black 96 Well Plate, 1EA

X025-1EA 1EA
EUR 19

Brand 96 Well Deep Well Plate-Stackable

BR701354-24EA PK24
EUR 163.2

Adapter for 96 well plate

3510147 1unit
EUR 6697.2

96 Well Skirted PCR Plate

PCR1236 PK50
EUR 385.2

96 Well Semi Skirted Plate

PCR1216 PK50
EUR 204

2 x Clear 96 well Plate, 2EA

X003-2EA 2EA
EUR 13

2 x Clear 96 well Plate, 2EA

X018-2EA 2EA
EUR 13

96 WELL PCR SEGMENTED PLATE

PCR-96-SG-C 10/pk
EUR 174
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

Tissue Culture Plate, 96 Well

TCP20-96 1 UNIT
EUR 64.18

DEEP WELL PLATE 96 WELL 2.0 ML STERIL

AXYPDW20CS PK50
EUR 240

384 WELL BLACK RIGI PLATE PCR MICROPLATE

PCR-384-RGD-BK 20/pk
EUR 585.6
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96 Well PCR Plate Clear Wells

PCR0972 PK50
EUR 258

96-Well Protein Folding Plate

PFS096 kit
EUR 347.84
Description: This product includes one 96-well plate with 96 protein folding solutions, 0.5 ml in each well of the mother plate; 1.4 ml of Inclusion Body Solubilizer; 4 ml of Neutralizer. Each experiment uses 0.1 ml of the solutions from the mother plate. Each mother plate contains 0.5 ml of solutions in each well and can be used for multiple experiments of folding various proteins.

96 Deep Well Plate Round Wells

PCR0242 PK50
EUR 292.8

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY

P-DW-12-HC 5/pk
EUR 182.4
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

Strip plates 96-well 12xF8 non dividable immunoGrade HB

F782305 PK100
EUR 642

Aligned Nanofibers 96 Well Plate

3D00012-96 700 nm-PCLs
EUR 132

96 Deep Well Plate Square Wells

PCR0240 PK100
EUR 336

96 Deep Well Plate Square WellU

PCR0256 PK50
EUR 232.8

Streptavidin coated 96-well Plate

6523-5 each
EUR 392.4

Wizard Cryo 1 and 2 96 Well Matrix Block Plate (96 conditions 1.7 mL volumes in a 96-Well block plate)

M-R-1008649 98 X 1.7 ml
EUR 316
Description: Wizard Cryo 1 and 2 96 Well Matrix Block Plate (96 conditions 1.7 mL volumes in a 96-Well block plate)

Nunc Lid for 96 well plate

264122 PK50
EUR 127.2

96 Well ABI Fast Plate Pcr

PCR1200 PK50
EUR 258

Corning Plate 8wl Strip Eia Fb Hb Ns

2580 PK800
EUR 572.4

PCR Plate Non Skirted 96 Well

MOL2364 PK50
EUR 309.6

96 Well Roche Style Pcr Plate

PCR1214 PK50
EUR 294

96 Well Roche Style PCR Plate

PCR1218 PK50
EUR 271.2

384 WELL CLEAR RIGI PLATE PCR MICROPLATE. CLEAR

PCR-384-RGD-C 20/pk
EUR 550.8
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

Clear Coated 96 Well Plate, 1EA

C107-1EA 1EA
EUR 162

PCR Plate Semi Skirted 96 Well

MOL2366 PK50
EUR 301.2

96 Well Roche Style Qpcr Plate

PCR1210 PK50
EUR 283.2

96 well filter plate (no filter)

SD5006-EMPTY 12UNIT
EUR 104.89

96 Well Skirted Plate+ Q-Stick

PCR1238 PK50
EUR 277.2

96 Well Solid Bottom Plate Black

PCR0354 PK100
EUR 422.4

96 Well Solid Bottom Plate White

PCR0360 PK100
EUR 388.8

96 Well PCR Plate Divisible In12

PCR1064 PK50
EUR 174

96 Well Semi-Skirted PCR Plate

PCR1106 PK50
EUR 368.4

Random Access 96 Well Plate Skir

PCR1412 PK50
EUR 301.2

S100 96-well PCR Plate Adapter

E5391070026 EACH
EUR 379.2

S200 96-well PCR Plate Adapter

E5392070038 EACH
EUR 379.2

Adapter for 96 well plate, pack of 2

IPD9600-96 each
EUR 1337.97

Nunc MicroWell 96 well polystyrene plates

P7366-1CS PK60
EUR 117.72

96 well DNA plate with membrane (960ul each well)

SD5007 12UNIT
EUR 291.24

96 well RNA plate with membrane (960ul each well)

SD5009 12UNIT
EUR 313.16

96 Deep Well Plate Round Wells U

PCR0228 PK100
EUR 217.2

96 Deep Well Plate Round Wells V

PCR0230 PK100
EUR 217.2

Strip plates with grid 96-well 12xF8 dividable immunoGrade HB

F782306 PK100
EUR 892.8

Adapter for 96-well PCR plate 2 pcs.

E5825711009 PK2
EUR 158.4

96 WELL BARCODED H1-H12, CLEAR PCR FULL SKIRT PCR AMPLIFICATION MICRO PLATE

PCR-96-FS-C-PBC 10/pk
EUR 201.6
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

BioMag® 96-Well Plate Separator

8MB4109S-1 1magnet
EUR 955

Nunc MicroWell 96-Well Black PS Plates

165305 PK30
EUR 790.8

INTELLI-PLATE 96-2 Shallow well;  2-well sitting drop, 120 plates

MAR-102-0001-20 120 PLATES
EUR 990
Description: INTELLI-PLATE 96-2 Shallow well;  2-well sitting drop, 120 plates

Wizard Classic 1 and 2 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate)

M-R-1008647 96 X 1.7 ml
EUR 316
Description: Wizard Classic 1 and 2 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate)

Nunc Lid for 96 Well Plate SH

263339 PK100
EUR 112.8

Analysis of human and canine Brucella canis an infection: growth and analysis of oblique enzyme-linked immunosorbent assays utilizing recombinant Brucella proteins.

Brucella canis, a Gram-negative coccobacilli belonging to the genus Brucellae, is a pathogenic bacterium that may produce infections in canines and people. A number of research have been carried out to develop diagnostic methods to detect all zoonotic Brucellae. Analysis of Brucella canis an infection is difficult as a result of lack of extremely particular and delicate diagnostic assays.

This work was divided in two phases: within the first one, had been recognized antigenic proteins in B. canis that would probably be used for serological analysis of brucellosis. Human sera optimistic for canine brucellosis an infection was used to acknowledge immunoreactive proteins that had been then recognized by performing 2D-GEL and immunoblot assays.

These spots had been analyzed utilizing MALDI TOF MS and predicted proteins had been recognized. Of the 35 protein spots analyzed, 14 proteins had been recognized and subsequently characterised utilizing bioinformatics, two of this had been chosen for the following section. Within the second section, we developed and validated an oblique enzyme-linked immunosorbent assays utilizing these recombinant proteins: inosine 5′ phosphate dehydrogenase, pyruvate dehydrogenase E1 subunit beta (PdhB) and elongation issue Tu (Tuf).

These genes had been PCR-amplified from genomic DNA of B. canis pressure Oliveri, cloned, and expressed in Escherichia coli. Recombinant proteins had been purified by metallic affinity chromatography, and used as antigens in oblique ELISA. Serum samples from wholesome and B. canis-infected people and canines had been used to guage the efficiency of oblique ELISAs. Our outcomes counsel that PdhB and Tuf proteins could possibly be used as antigens for serologic detection of B. canis an infection in people, however not in canines.

The usage of recombinant antigens in iELISA assays to detect B. canis-specific antibodies in human serum could possibly be a beneficial instrument to enhance analysis of human brucellosis attributable to B. canis.

Key phrases: Micro organism; Bacteriology; Organic sciences; Brucella canis str. Oliveri; Brucellosis; ELISA check; Well being sciences; Immunoproteome: people; Infectious illness; Microbiology; Microorganism; Proteins; Proteomics; Veterinary drugs; Zoonoses.

Neptune™ Block ELISA Blocking Buffer

64 1 L
EUR 328

Blocking Buffer

abx098972-1vial 1 vial
EUR 184.8

Blocking Buffer

RM01757 20mL
EUR 280

Alternative Block™ ELISA Blocking Buffer, Synthetic

6299 100 mL
EUR 52.25

Alternative Block™ ELISA Blocking Buffer, Synthetic

6300 500 mL
EUR 145

Alternative Block™ ELISA Blocking Buffer, Synthetic

6301 1 L
EUR 290

OOMA00026-500ML - Blocking Buffer for ELISA

OOMA00026-500ML 500ML
EUR 349

ELISA Plate Blocking Buffer concentrate (10X) BSA-based

80060 50 ml
EUR 242.4

Monster Block™ ELISA Blocking Buffer, Non-Mammalian

6295 100 mL
EUR 52.25

Monster Block™ ELISA Blocking Buffer, Non-Mammalian

6296 500 mL
EUR 145

Monster Block™ ELISA Blocking Buffer, Non-Mammalian

6297 1 L
EUR 290

TBS Blocking Buffer Pack

AR0143 200mL/pack
EUR 78

Blocking Buffer, 10X, 250ML

X109-250ML 250ML
EUR 360

Blocking Buffer, 10X, 25ML

X109-25ML 25ML
EUR 59

Phosph-Free Blocking Buffer, Synthetic

6262 100 mL
EUR 52.25

Phosph-Free Blocking Buffer, Synthetic

6263 500 mL
EUR 159

Phosph-Free Blocking Buffer, Synthetic

6264 1 L
EUR 290

General Blocker Blocking Buffer

632 100 mL
EUR 45.6

General Blocker Blocking Buffer

633 500 mL
EUR 143.5

General Blocker Blocking Buffer

640 1 L
EUR 260.5

EZBlock? (TBS) Blocking Buffer

2117-1000 each
EUR 254.4

EZBlock? (TBS) Blocking Buffer

2117-200 each
EUR 150

EZBlock? (PBS) Blocking Buffer

2128-1000 each
EUR 274.8

EZBlock? (PBS) Blocking Buffer

2128-200 each
EUR 164.4

General Blocking Buffer-BB1

KF17337 500 ml
EUR 277.2

Neptune Blocking Buffer-BB2

KF17338 500 ml
EUR 292.8

OORA01662-1U - Blocking Buffer

OORA01662-1U 1Each
EUR 630

ChonBlock Blocking/Sample Dilution ELISA Buffer, 100 ml

9068 100 ml
EUR 155.7
Description: ChonBlock Blocking/Sample Dilution ELISA Buffer

OORA01662-1EA - Blocking Buffer

OORA01662-1EA 1Each
EUR 569

OORA01673-10U - Blocking Buffer

OORA01673-10U 1Each
EUR 1735

OORA01673-1EA - Blocking Buffer

OORA01673-1EA 1Each
EUR 1569

OORA01674-10U - Blocking Buffer

OORA01674-10U 1Each
EUR 2375

OORA01674-1EA - Blocking Buffer

OORA01674-1EA 1Each
EUR 2139

PBS Blocking buffer with BSA

SB0626 1pk, 1L
EUR 100.72

TBS blocking buffer with BSA

SD8113 1pk, 1L
EUR 100.72

OORA01675-50ML - Blocking Buffer

OORA01675-50ML 50mL
EUR 79

Casein Blocking Buffer in PBS

40320020-1 500 mL
EUR 41.24

Casein Blocking Buffer in PBS

40320020-2 1 L
EUR 69.42

Casein Blocking Buffer in TBS

40320028-1 500 mL
EUR 34.05

Casein Blocking Buffer in TBS

40320028-2 1 L
EUR 57.9

OORA01665-500ML - Blocking Buffer

OORA01665-500ML 500mL
EUR 229

OORA01666-100ML - Blocking Buffer

OORA01666-100ML 100mL
EUR 179

3 Blocking Buffer Sample Pack

KF17340 3 x 100 ml
EUR 246

EZBlock? T20 (TBS) Blocking Buffer

2140-1000 each
EUR 261.6

EZBlock? T20 (TBS) Blocking Buffer

2140-200 each
EUR 144

EZBlock? T20 (PBS) Blocking Buffer

2143-1000 each
EUR 261.6

EZBlock? T20 (PBS) Blocking Buffer

2143-200 each
EUR 144

10 x Universal Blocking Buffer

GR103005 200 mL
EUR 129

TrueBlack WB Blocking Buffer Kit

23013 1kit
EUR 416.4
Description: Minimum order quantity: 1 unit of 1kit

TrueBlack WB Blocking Buffer Kit

23013-T 1kit
EUR 150
Description: Minimum order quantity: 1 unit of 1kit

Corning 3D Clear Blocking Buffer - 100mL

5737 EACH
EUR 325.2

Blocking Buffer for ICC and IHC

SF40011 10 ml
EUR 153.6

West-Ezier Super Blocking Buffer

W3700-010 100ml
EUR 116.4

West-Ezier Super Blocking Buffer

W3700-050 500ml
EUR 198

West-Ezier Super Blocking Buffer

W3700-100 1L
EUR 295.2

OORA00238-1L - ELISA Microwell Blocking Buffer with Stabilizer

OORA00238-1L 1L
EUR 319

Gelatin (1%) Blocking Buffer in PBS

40720012-1 500 mL
EUR 29.83

Gelatin (1%) Blocking Buffer in PBS

40720012-2 1 L
EUR 56.78

Gelatin (1%) Blocking Buffer in TBS

40720020-1 500 mL
EUR 29.83

Gelatin (1%) Blocking Buffer in TBS

40720020-2 1 L
EUR 53.99

BSA (3%) Blocking Buffer 1X in TBS

40220095-1 100 mL
EUR 30.47

BSA (3%) Blocking Buffer 1X in TBS

40220095-2 250 mL
EUR 44.66

BSA (3%) Blocking Buffer 1X in TBS

40220095-3 1 L
EUR 77.39

BSA (3%) Blocking Buffer 1X in PBS

21420071-1 250 ml
EUR 31.63

BSA (3%) Blocking Buffer 1X in PBS

21420071-2 500 ml
EUR 49.18

BSA (3%) Blocking Buffer 1X in PBS

21420071-3 1 L
EUR 73.3

Blocking Buffer and Coating Stabilizer

abx090811-1000ml 1000 ml
EUR 627.6

TrueBlack® WB Blocking Buffer Kit

23013-T-1 KT
EUR 89

OORA00239-100ML - ELISA Microwell Blocking Buffer with Stabilizer

OORA00239-100ML 100mL
EUR 119

BSA (3%) Blocking Buffer in 10X PBS

40220064-1 100 mL
EUR 33.17

BSA (3%) Blocking Buffer in 10X PBS

40220064-2 200 mL
EUR 49.75

BSA (3%) Blocking Buffer in 10X PBS

40220064-3 500 mL
EUR 112.92

BSA (3%) Blocking Buffer in PBST 1X

21420072-1 250 ml
EUR 25.12

BSA (3%) Blocking Buffer in PBST 1X

21420072-2 500 ml
EUR 43.6

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