elisareagents

Analysis of an in-house oblique enzyme-linked immunosorbent assay of feline panleukopenia VP2 subunit antigen compared to hemagglutination inhibition assay to watch tiger antibody ranges by Bayesian strategy

Background: Feline panleukopenia virus (FPV) is an etiologic pathogen of feline panleukopenia that infects all members of Felidae together with tigers (Panthera tigris). Vaccinations in opposition to FPV amongst wild felid species have lengthy been practiced in zoos worldwide.
Nevertheless, few research have assessed the tiger immune response post-vaccination because of the absence of a serological diagnostic instrument. To deal with these limitations, this examine aimed to develop an in-home oblique enzyme-linked immunosorbent assay (ELISA) for the monitoring of tiger antibody ranges in opposition to the feline panleukopenia vaccine by using the synthesized subunit capsid protein VP2.
An in-house horseradish peroxidase (HRP) conjugated rabbit anti-tiger immunoglobulin G (IgG) polyclonal antibody (HRP-anti-tiger IgG) was produced on this examine and employed within the assay. It was then in comparison with a business HRP-conjugated goat anti-cat IgG (HRP-anti-cat IgG). Sensitivity and specificity have been evaluated utilizing the Bayesian mannequin with preferential conditional dependence between HRP-conjugated antibody-based ELISAs and hemagglutination-inhibition (HI) checks.
Outcomes: The posterior estimates for sensitivity and specificity of two oblique ELISA HRP-conjugated antibodies have been increased than these of the HI take a look at. The sensitivity and specificity of the oblique ELISA for HRP-anti-tiger IgG and HRP-anti-cat IgG have been 86.5, 57.2 and 86.7%, 64.6%, respectively, whereas the outcomes of the HI take a look at have been 79.1 and 54.1%. In purposes, 89.6% (198/221) and 89.1% (197/221) of the tiger serum samples have been decided to be seropositive by oblique ELISA testing in opposition to HRP-anti-tiger and HRP-anti-cat, respectively.
Conclusion: To one of the best of our information, the particular serology assays for the detection of the tiger IgG antibody haven’t but been established. The HRP-anti-tiger IgG has been produced for the aim of creating the particular immunoassays for tigers. Remarkably, an in-house oblique ELISA based mostly on VP2 subunit antigen has been efficiently developed on this examine, offering a probably worthwhile serological instrument for the efficient detection of tiger antibodies.
Key phrases: Bayesian mannequin; Capsid protein VP2; Feline panleukopenia virus; Hemagglutination inhibition assay; Oblique ELISA; Tiger (Panthera tigris).
elisareagents
elisareagents

Crystallization Plate Sealing Paddle Qty: 5

M-CTP-PDL1 5 PADDLES
EUR 21
Description: Crystallization Plate Sealing Paddle Qty: 5

Optical Sealing Film96 well PCR plate F

PR18-1X100NO 1 unit
EUR 236.8
Description: Optical Sealing Film96 well PCR plate F

PIERCABLE SEALING MAT FOR 24 WELL DEEP WELL PLATE

AM-24-SQ 10/pk
EUR 248.4
Description: Sealing Products; Sealing mats - Axygen

PIERCABLE SEALING MAT FOR 24 WELL DEEP WELL PLATE, STERILE

AM-24-SQ-S 10/pk
EUR 280.8
Description: Sealing Products; Sealing mats - Axygen

Kinesis Silicone Round Well Sealing Mat 500ul plate Slit - PK5

CHR1239 PK5
EUR 76.95

Axygen 384 well white PCR plate for Roche 480 LightCycler no sealing - PK10

AXY2090 PK10
EUR 85.05

Axygen 96 well white PCR plate for Roche 480 LightCycler no sealing - PK10

AXY2112 PK10
EUR 67.5

Optically clear sealing film for qPCR, Peelable, for most plate types, 100/pk

MS1000-PCR1 1 each
EUR 149.35

Optically clear sealing film for qPCR, Pierceable, for most plate types, 100/pk

MS1000-PCR3 1 each
EUR 149.35

IntelliXseal SA Weighted Sealing Platen - EA

59-2008 EA
EUR 102.6

Axygen 384 well white PCR plate for Roche 480 LightCycler with UC-500 sealing - PK10

AXY2086 PK10
EUR 149.85

SEALING FILM ROLLER, FOR APPLYING PLATEMAX™ SEALING FILMS, WITH STURDY PLASTIC HANDLE AND SEMI-HARD ROLLER HEAD

PCR-SP-ROLLER 1/pk
EUR 72
Description: Sealing Products; Sealing films - Axygen

AgileSealerTM Semi Automated Plate Sealer (including 1 adaptor for 96-well PCR plate, 1 adaptor for flexible plates, and 1 weighted sealing flake)

APS-200 each
EUR 2800

Sealing Film For Plates PP/PE With Adhesive - PK50

701370 PK50
EUR 307.8

Axygen 96 well bar-coded white PCR plate for Roche 480 LightCycler with UC-500 sealing - PK50

AXY2110 PK50
EUR 623.7

Kinesis Silicone Round Well Sealing Mat 1.1ml Plates; Slit - PK5

CHR1237 PK5
EUR 64.8

PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500

HS-150-QPCR 100/pk
EUR 381.6
Description: Sealing Products; Sealing films - Axygen

PLATEMAX PEELABLE HEAT SEALING FILM FOR COMPOUND STORAGE. 100/500

HS-400 100/pk
EUR 364.8
Description: Sealing Products; Sealing films - Axygen

PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500

HS-100-QPCR 100/pk
EUR 381.6
Description: Sealing Products; Sealing films - Axygen

Crystallization Plate Support Clear acrylic support block that fits under InSitu, CD1, CD2 plates. Primary use to support plate center when applying sealing film.

M-CTP-PSS-1 1 Unit
EUR 39
Description: Crystallization Plate SupportClear acrylic support block that fits under InSitu, CD1, CD2 plates. Primary use to support plate center when applying sealing film.

Optically clear sealing film for qPCR, Strong Bond, for PP plates, 100/pk

MS1000-PCR2 1 each
EUR 149.35

UVXPO Sealing Sheets

CTP-106 100pcs.
EUR 323.38

Sealing plugs GE - PK5

19003901 PK5
EUR 85.05

PLATEMAX PIERCEABLE ALUMINUM HEAT SEALING FILM FOR STORAGE APPLICATIONS. 100/500

HS-300 100/pk
EUR 364.8
Description: Sealing Products; Sealing films - Axygen

TAPE-SEQUENCING SEALING

IB81630 -
EUR 60.28

SafeStore Silicone Sealing Mat for 96 Round Well 1.0ml plates, piercable - 50 mats

WB0566 each
EUR 296.48

SafeStore Silicone Sealing Mat for 96 Round Well 2.0ml plates, piercable - 50 mats

WB0567 each
EUR 296.48

POLYPROPYLENE SEALING FILM

PR21-1X100NO 1 unit
EUR 133.88
Description: POLYPROPYLENE SEALING FILM

Sealing Film, 50 Sheets

TB418 1PK, 50UNIT
EUR 73.57

Sealing Ring Svl15 - PK10

701-01 PK10
EUR 76.95

Axymat Silicone Sealing Mat

CCM-103 10pcs.
EUR 95.84

SEALING FILM, PARAFILM, 2x250

S801 12EA
EUR 51.54

SEALING FILM, PARAFILM; 4x125

S823 12EA
EUR 34.66

SEALING FILM, PARAFILM, 4x250

S911 10EA
EUR 71.79

Sealing Film Polyester - PK100

MOL2374 PK100
EUR 318.6

PLATEMAX PEELABLE HEAT SEALING FILM FOR LOW TEMPERATURE COMPOUND STORAGE AND PCR. 100/500

HS-200 100/pk
EUR 364.8
Description: Sealing Products; Sealing films - Axygen

Sealing Film Polypropylene - PK100

MOL2372 PK100
EUR 229.5

Clear Heat Sealing Seals (100sheets)

MAAB-0812 each
EUR 190.75

Sealing Film, Sterile, 50 Sheets

TB418-S 1PK, 50UNIT
EUR 74.62

Eppendorf Heat Sealing Film - EACH

E0030127838 EACH
EUR 232.2

Sealing Film, Foil Tight Seal, 100Sheet

SMT4406 1PK, 100UNIT
EUR 247.92

Eppendorf Heat Sealing Foil - PK100

E0030127854 PK100
EUR 164.7

Sealing film for Automation - PK100

MOL2258 PK100
EUR 225.45

Nunc Sealing Tape Polyolefin - PK100

235307 PK100
EUR 777.6

DURAN Silicone Sealing Gasket - PK10

BOT2280 PK10
EUR 51.3

SEALING MAT, FOR 1.1 AND 2 ML 96 WELL DEEP WELL PLATES, ROUND WELLS, SILICONE, NATURAL, NONSTERILE, BULK

AM-2ML-RD 10/pk
EUR 196.8
Description: Sealing Products; Sealing mats - Axygen

Sealing Cap Nalgene (250/290Ml) - PK2

CEN9034 PK2
EUR 143.1

Sealing Caps For Cen9526/36 - PK2

CEN9544 PK2
EUR 48.6

Sealing Mats for 96 Well Format

GP501 5 mats
EUR 176.4

Sealing Mat for DWP 96/2000 - PK50

E0030127960 PK50
EUR 199.8

96 Well MPlate Sealing Strip - PK24

CHR5886 PK24
EUR 79.65

Heat Seals ALPS Microplate Sealing - PK100

AB0559 PK100
EUR 168.75

Replacement sealing ring, (GL45), 10/pk.

B3000-RIN 1 PC
EUR 14.94

Replacement sealing ring, (GL32), 10/pk.

B3000-RIN2 1 PC
EUR 14.94

Hettich Sealing lid 250ml Bucket - EACH

CEN0717 EACH
EUR 190.35

Sealing film, Aluminum membrane, pk/100

P1001-A 100/pack
EUR 82.68
Description: Sealing film Aluminum membrane

Boot Covers - PK200

SAF0340 PK200
EUR 525.15

Sealing Mat (Round Cap Pierceable) - PK50

AB-0566 PK50
EUR 282.15

Peel-seal Heat Sealing Sheets - PK100

PCR0580 PK100
EUR 122.85

Sealing Mats round cap autoclavable (50)

AB-0674 each
EUR 162.41

Kinesis Sealing Ring; 1/16; PTFE - PK20

CHR1847 PK20
EUR 72.9

Easy Pierce 20µm Heat Sealing Foil (100)

MAAB-1720 each
EUR 117.18

Nunc Sealing Tape 134x80mm Sterile - PK200

236366K PK200
EUR 156.6

Sealing Mats square cap autoclavable (50)

AB-0675 each
EUR 162.41

Axygen Aluminium sealing film 35um - PK500

AXY2132 PK500
EUR 808.65

Axygen Aluminium sealing film 50um - PK500

AXY2250 PK500
EUR 703.35

sealing Agent, 50 mL/vial, 4 vials/cs

208221 40 pcs/cs
EUR 160.49
Description: sealing Agent, 50 mL/vial, 4 vials/cs

sealing Agent, 50 mL/vial, 4 vials/cs

211121 4 pcs/cs
EUR 149.82
Description: sealing Agent, 50 mL/vial, 4 vials/cs

Kinesis Sealing Ring Graphite Thermo - PK10

CHR1816 PK10
EUR 116.1

Sealing Caps For Cen9522/24/32/34 - PK2

CEN9542 PK2
EUR 40.5

Sealing Mat for DWP 96/1000 or /500 - PK50

E0030127978 PK50
EUR 199.8

Nunc polyolifin Sealing Tape 121x79mm - PK100

232701 PK100
EUR 256.5

SEALING TAPE APPLICATOR MANUAL; 2/PK - PK2

60180-M950 PK2
EUR 37.8

Hettich Sealing lid for 250ml bucket - EACH

CEN0622 EACH
EUR 153.9

Pressure Sealing Mats for 384 Well Format

GP504 5 mats
EUR 235.2

PCR Sealing Film, 146 × 81 mm, 100/pk, 500/cs

410001 500 pcs/cs
EUR 127.38
Description: PCR Sealing Film, 146 × 81 mm, 100/pk, 500/cs

PCR Sealing Film, 141 × 78 mm, 100/pk, 500/cs

410011 500 pcs/cs
EUR 669.24
Description: PCR Sealing Film, 141 × 78 mm, 100/pk, 500/cs

Technical Observe: Utilizing enzyme-linked immunosorbent assays to judge humoral responses to vaccination in opposition to respiratory viruses in beef cattle

This experiment evaluated humoral responses in beef calves vaccinated in opposition to parainfluenza-Three virus (PI3), bovine respiratory syncytial virus (BRSV), and bovine herpesvirus-1 (BHV-1) utilizing by way of serum neutralization (SN) checks or enzyme-linked immunosorbent assays (ELISA). Blood samples have been collected from 50 overtly wholesome Angus-influenced steers (183 ± Three kg of physique weight, 212 ± 2 d of age) on d 0, 21, 35, and 49 of the experiment. Steers have been vaccinated in opposition to respiratory viruses on d Zero and 21.
Blood was processed for serum assortment and frozen in duplicates. One of many duplicates was analyzed for antibodies in opposition to BRSV, PI3, and BHV-1 utilizing commercially accessible ELISA (IDEXX Switzerland AG, Liebefeld-Bern, Switerland), and outcomes reported as pattern:constructive management (S/P, %) ratio. The opposite duplicate was analyzed for antibodies in opposition to the identical vaccine antigens by way of SN.
This methodology report outcomes as titers, the best dilution that gives full safety of the cells, which have been remodeled with base 2 log for statistical analyses. Samples have been categorized as constructive for presence of antibodies by SN if log-transformed titer ≥ 2 for all viruses, and by ELISA if S/P ratio ≥ 50% for BHV-1 or ≥ 20% for PI3 and BRSV. Day results have been detected (P < 0.01) for SN and ELISA throughout all vaccine antigens, as antibody ranges elevated after vaccine administration.
Linear suits have been detected (P < 0.01) throughout all vaccine antigens when regressing the SN and ELISA outcomes; as SN titer elevated the ELISA S/P ratio linearly elevated (P < 0.01). Kendall (τ) and Spearman’s rank (ρ) correlations have been additionally detected (P < 0.01) between SN and ELISA outcomes throughout all vaccine antigens. The SN and ELISA have been very strongly correlated (ρ ≥ 0.83) for BHV-1 and PI3, and strongly correlated (ρ = 0.66) for BRSV. Cohen’s kappa coefficient for analysis settlement between strategies have been robust for BHV-1 and PI3 (κ ≥ 0.88), however weak (κ = 0.47) for BRSV.
The sensitivity of the ELISA in yielding true constructive outcomes approached 100% throughout all antigens. The specificity of the ELISA in yielding unfavorable outcomes have been passable for BHV-1 and PI3 assays (84.Zero and 88.5%, respectively) however not for BRSV (34.4%).
Regardless of limitations in detecting true BRSV negatives, outcomes from this experiment point out that the business ELISAs examined herein can be utilized as surrogate for SN checks in quantifying humoral responses to vaccination in opposition to BHV-1, PI3, and BRSV in beef cattle.
Key phrases: beef cattle; humoral response; respiratory illness; vaccination

Alternative Block ELISA Blocking Buffer, Synthetic

MBS258395-500mL 500mL
EUR 230

Alternative Block ELISA Blocking Buffer, Synthetic

MBS258395-5x1L 5x1L
EUR 1435

Monster Block ELISA Blocking Buffer, Non-Mammalian

MBS258394-100mL 100mL
EUR 160

Monster Block ELISA Blocking Buffer, Non-Mammalian

MBS258394-1L 1L
EUR 345

Monster Block ELISA Blocking Buffer, Non-Mammalian

MBS258394-500mL 500mL
EUR 230

Monster Block ELISA Blocking Buffer, Non-Mammalian

MBS258394-5x1L 5x1L
EUR 1435

Alternative Block™ ELISA Blocking Buffer, Synthetic

6299 100 mL
EUR 52.25

Alternative Block™ ELISA Blocking Buffer, Synthetic

6300 500 mL
EUR 145

Alternative Block™ ELISA Blocking Buffer, Synthetic

6301 1 L
EUR 290

Monster Block™ ELISA Blocking Buffer, Non-Mammalian

6295 100 mL
EUR 52.25

Monster Block™ ELISA Blocking Buffer, Non-Mammalian

6296 500 mL
EUR 145

Monster Block™ ELISA Blocking Buffer, Non-Mammalian

6297 1 L
EUR 290

OOSA11206-1L - ELISA NEPTUNE BUFFER Accessory Reagent

OOSA11206-1L 1000ml
EUR 749

OOSA09562-100ML - ELISA NEPTUNE BUFFER Accessory Reagent

OOSA09562-100ML 100ml
EUR 169

OOSA11205-500ML - ELISA NEPTUNE BUFFER Accessory Reagent

OOSA11205-500ML 500ml
EUR 519

Blocking Buffer for ELISA

abx090807-500ml 500 ml
EUR 460.8

Blocking Buffer for ELISA

GWB-E120C6 500 ml Ask for price

Blocking Buffer for ELISA

MBS318404-500mL 500mL
EUR 335

Blocking Buffer for ELISA

MBS318404-5x500mL 5x500mL
EUR 1315

ELISA Plate Blocking Buffer

E-ELIR-003-24Tests 24 Tests Ask for price

ELISA Plate Blocking Buffer

E-ELIR-003-48Tests 48 Tests
EUR 110

ELISA Plate Blocking Buffer

E-ELIR-003-96Tests 96 Tests
EUR 35

ELISA Plate Blocking Buffer

E-ELIR-003-96Tests10 96 Tests *10
EUR 350

ELISA Plate Blocking Buffer

E-ELIR-003-96Tests5 96 Tests *5
EUR 175

ELISA Plate Blocking Buffer

MBS2571969-100mL 100mL
EUR 110

ELISA Plate Blocking Buffer

MBS2571969-1L 1L
EUR 165

PanBlock ELISA Blocking Buffer

BUF81201-100 each
EUR 81.12
Description: Blocking Buffer for antigen-down and sandwich ELISA. PanBlock is for antigen-down and sandwich ELISAs and for those with high background problems or cross-react with mammalian buffers.

PanBlock ELISA Blocking Buffer

BUF81201-1000 each
EUR 384.98
Description: Blocking Buffer for antigen-down and sandwich ELISA. PanBlock is for antigen-down and sandwich ELISAs and for those with high background problems or cross-react with mammalian buffers.

SynBlock ELISA Blocking Buffer

MBS258400-100mL 100mL
EUR 160

SynBlock ELISA Blocking Buffer

MBS258400-1L 1L
EUR 345

SynBlock ELISA Blocking Buffer

MBS258400-500mL 500mL
EUR 245

SynBlock ELISA Blocking Buffer

MBS258400-5x1L 5x1L
EUR 1435

Blocking Buffer

abx098972-100l 100 µl
EUR 93.75

Blocking Buffer

abx098972-1ml 1 ml Ask for price

Blocking Buffer

abx098972-1vial 1 vial
EUR 184.8

Blocking Buffer

abx098972-200l 200 µl Ask for price

Blocking Buffer

CBB101 60 ml
EUR 87

Blocking buffer

IBS-BB005 500 mL
EUR 49

Blocking Buffer

ML044-100ML 1 unit
EUR 4.75
Description: Blocking Buffer

Blocking Buffer

ML044-500ML 1 unit
EUR 16.62
Description: Blocking Buffer

Blocking Buffer

RM01757 20mL
EUR 9.21

SynBlock™ ELISA Blocking Buffer

642 500 mL
EUR 159.25

SynBlock™ ELISA Blocking Buffer

643 1 L
EUR 290.5

Synthetic Blocking Buffer-for ELISA

4520A 1 L
EUR 150

Synthetic Blocking Buffer-for ELISA

4520L 500 ML
EUR 111

5% Blocking Buffer

E-IR-R107-10mL 10mL
EUR 10

5% Blocking Buffer

E-IR-R107-50mL 50mL
EUR 30

5% Blocking Buffer

E-IR-R107-5mL 5mL
EUR 8

5% Blocking Buffer

E-IR-R107-each each Ask for price

Western blocking buffer

IBS-BW005 100 mL
EUR 24.5

Western blocking buffer

IBS-BW005a 500 mL
EUR 70

Western Blocking Buffer

EZWB04-4-100mL 100mL
EUR 10.8

Western Blocking Buffer

EZWB04-4-500mL 500mL
EUR 38.4

Western Blocking Buffer

MBS355690-100mL 100mL
EUR 180

Western Blocking Buffer

MBS355690-5x100mL 5x100mL
EUR 500

OOMA00026-500ML - Blocking Buffer for ELISA

OOMA00026-500ML 500ML
EUR 349

General Blocker Blocking Buffer

632 100 mL
EUR 45.6

General Blocker Blocking Buffer

640 1 L
EUR 260.5

General Blocker Blocking Buffer

MBS258397-100mL 100mL
EUR 150

General Blocker Blocking Buffer

MBS258397-1L 1L
EUR 320

General Blocker Blocking Buffer

MBS258397-500mL 500mL
EUR 230

General Blocker Blocking Buffer

MBS258397-5x1L 5x1L
EUR 1325

Blocking Buffer for WB

GWB-41822F 500 ml Ask for price

5% BSA Blocking Buffer

MBS2567133-10mL 10mL
EUR 80

5% BSA Blocking Buffer

MBS2567133-50mL 50mL
EUR 95

5% BSA Blocking Buffer

MBS2567133-5mL 5mL
EUR 80

5% BSA Blocking Buffer

MBS2567133-5x50mL 5x50mL
EUR 390

T-Pro Blocking Buffer

JK92-W005 500ml/BT
EUR 60

TBS Blocking Buffer Pack

AR0143 200mL/pack
EUR 78

Blocking Buffer, 10X, 250ML

X109-250ML 250ML
EUR 360

Blocking Buffer, 10X, 25ML

X109-25ML 25ML
EUR 59

10X Casein Blocking Buffer

IBS-BC052 1L
EUR 105

EZBlock? (PBS) Blocking Buffer

2128-1000 each
EUR 274.8

EZBlock? (PBS) Blocking Buffer

2128-200 each
EUR 164.4

EZBlock? (TBS) Blocking Buffer

2117-1000 each
EUR 254.4

EZBlock? (TBS) Blocking Buffer

2117-200 each
EUR 150

ELISA Plate Blocking Buffer concentrate (10X) BSA-based

80060 50 ml
EUR 242.4

General Blocking Buffer-BB1

KF17337 500 ml
EUR 277.2

SynBlock Blocking Buffer-BB3

KF17339 500 ml
EUR 292.8

OORA01662-1U - Blocking Buffer

OORA01662-1U 1Each
EUR 630

PBS Blocking buffer with BSA

SB0626 1pk, 1L
EUR 100.72

TBS blocking buffer with BSA

SD8113 1pk, 1L
EUR 100.72

OORA01662-1EA - Blocking Buffer

OORA01662-1EA 1Each
EUR 569

OORA01673-10U - Blocking Buffer

OORA01673-10U 1Each
EUR 1735

OORA01673-1EA - Blocking Buffer

OORA01673-1EA 1Each
EUR 1569

OORA01674-10U - Blocking Buffer

OORA01674-10U 1Each
EUR 2375

OORA01674-1EA - Blocking Buffer

OORA01674-1EA 1Each
EUR 2139

Casein Blocking Buffer in PBS

40320020-1 500 mL
EUR 54.82

Casein Blocking Buffer in PBS

40320020-2 1 L
EUR 86.22

Casein Blocking Buffer in TBS

40320028-1 500 mL
EUR 52.79

Casein Blocking Buffer in TBS

40320028-2 1 L
EUR 86.19

OORA01675-50ML - Blocking Buffer

OORA01675-50ML 50mL
EUR 79

10× Normal Goat Blocking Buffer

E-IR-R111-10mL 10mL
EUR 20

10× Normal Goat Blocking Buffer

E-IR-R111-5mL 5mL
EUR 15

10× Normal Goat Blocking Buffer

E-IR-R111-each each Ask for price

3 Blocking Buffer Sample Pack

KF17340 3 x 100 ml
EUR 246

OORA01665-500ML - Blocking Buffer

OORA01665-500ML 500mL
EUR 229
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