elisareagents

Analysis of an in-house oblique enzyme-linked immunosorbent assay of feline panleukopenia VP2 subunit antigen compared to hemagglutination inhibition assay to watch tiger antibody ranges by Bayesian strategy

Background: Feline panleukopenia virus (FPV) is an etiologic pathogen of feline panleukopenia that infects all members of Felidae together with tigers (Panthera tigris). Vaccinations in opposition to FPV amongst wild felid species have lengthy been practiced in zoos worldwide.
Nevertheless, few research have assessed the tiger immune response post-vaccination because of the absence of a serological diagnostic instrument. To deal with these limitations, this examine aimed to develop an in-home oblique enzyme-linked immunosorbent assay (ELISA) for the monitoring of tiger antibody ranges in opposition to the feline panleukopenia vaccine by using the synthesized subunit capsid protein VP2.
An in-house horseradish peroxidase (HRP) conjugated rabbit anti-tiger immunoglobulin G (IgG) polyclonal antibody (HRP-anti-tiger IgG) was produced on this examine and employed within the assay. It was then in comparison with a business HRP-conjugated goat anti-cat IgG (HRP-anti-cat IgG). Sensitivity and specificity have been evaluated utilizing the Bayesian mannequin with preferential conditional dependence between HRP-conjugated antibody-based ELISAs and hemagglutination-inhibition (HI) checks.
Outcomes: The posterior estimates for sensitivity and specificity of two oblique ELISA HRP-conjugated antibodies have been increased than these of the HI take a look at. The sensitivity and specificity of the oblique ELISA for HRP-anti-tiger IgG and HRP-anti-cat IgG have been 86.5, 57.2 and 86.7%, 64.6%, respectively, whereas the outcomes of the HI take a look at have been 79.1 and 54.1%. In purposes, 89.6% (198/221) and 89.1% (197/221) of the tiger serum samples have been decided to be seropositive by oblique ELISA testing in opposition to HRP-anti-tiger and HRP-anti-cat, respectively.
Conclusion: To one of the best of our information, the particular serology assays for the detection of the tiger IgG antibody haven’t but been established. The HRP-anti-tiger IgG has been produced for the aim of creating the particular immunoassays for tigers. Remarkably, an in-house oblique ELISA based mostly on VP2 subunit antigen has been efficiently developed on this examine, offering a probably worthwhile serological instrument for the efficient detection of tiger antibodies.
Key phrases: Bayesian mannequin; Capsid protein VP2; Feline panleukopenia virus; Hemagglutination inhibition assay; Oblique ELISA; Tiger (Panthera tigris).
elisareagents
elisareagents

Sealing Film, 50 Sheets

TB418 1PK, 50UNIT
EUR 61.31

dummy plate

EVS3100-DUMMY ea
EUR 48

Cover Plate

1292235 5unit
EUR 251

Plate Roller

TSM113 1 UNIT
EUR 55.22

Plate Rotator

abx725037-1Unitwith1215ml450mlplate 1 Unit with 12 × 15 ml 4 × 50 ml plate
EUR 589

Plate Rotator

abx725037-1Unitwith1215ml450mlplate2215mlplate 1 Unit with 12 × 15 ml 4 × 50 ml plate + 22 × 15 ml plate
EUR 624

SEALING TAPE, BREATHABLE, STERILE, BULK

3345 50/pk
EUR 412
Description: Storage; Sealing Tapes

Sealing Film, Sterile, 50 Sheets

TB418-S 1PK, 50UNIT
EUR 62.18

Sample Treatment Plate

F402 1x 96 wells plate
EUR 103
Description: Sample Treatment Plate by Cygnus Technologies is available in Europe via Gentaur.

notched aluminium plate

EVS3100-ALU ea
EUR 48

glass plate 10x10cm

EVS3100-GLASS ea
EUR 48

glass plate 20x10cm

EVS3200-GLASS ea
EUR 56

glass plate 20x20cm

EVS3300-GLASS ea
EUR 56

2 stair plate

2312210 1unit
EUR 264

Dummy plate 1pc

2322215 10unit
EUR 257

Plate Set, CP

2393634 4unit
EUR 260
Description: 1 pai r of not ched & pl ai n gl ass pl at es

Dummy Plate, CP

2394060 10unit
EUR 272
Description: For r unni ng 1 gel onl y

Micro-Plate Washer

abx700010-1Unit 1 Unit
EUR 4382

Plate Thaw Station

abx725013-1Unit 1 Unit
EUR 328

Hot Plate Stirrer

abx725020-1Unitwith10Positions 1 Unit with 10 Positions
EUR 1546

Hot Plate Stirrer

abx725020-1Unitwith15Positions 1 Unit with 15 Positions
EUR 1859

Hot Plate Stirrer

abx725020-1Unitwith5Positions 1 Unit with 5 Positions
EUR 1163

Digital Plate Rotator

abx725036-1Unitwith1650mlTubePlate 1 Unit with 16 × 50 ml Tube Plate
EUR 885

Digital Plate Rotator

abx725036-1Unitwith1650mlTubePlate552mlTubePlate 1 Unit with 16 × 50 ml Tube Plate + 55 × 2ml Tube Plate
EUR 989

Digital Plate Rotator

abx725036-1Unitwith3515mlTubePlate 1 Unit with 35 × 15 ml Tube Plate
EUR 885

Digital Plate Rotator

abx725036-1Unitwith3515mlTubePlate552mlTubePlate 1 Unit with 35 × 15 ml Tube Plate + 55 × 2ml Tube Plate
EUR 1007

Plate Sealer, 1EA

X002-1EA 1EA
EUR 53

Plate Primer, 25ML

X073-25ML 25ML
EUR 102

96-Well Plate

1996-2
EUR 104

Weighted sealing flake, accessory for AgileSealer?

APS-200-SF
EUR 351

Sealing Film, Foil Tight Seal, 100Sheet

SMT4406 1PK, 100UNIT
EUR 206.6

Sealing film, Aluminum membrane, pk/100

P1001-A 100/pack
EUR 95.9
Description: Sealing film Aluminum membrane

Sealing Mats for 96 Well Format

GP501 5 mats
EUR 147

OxiSelect Nitrotyrosine ELISA Kit (1 plate)

STA-305 96 assays
EUR 798
Description: Nitric oxide influences a variety of biological processes including cell proliferation, apoptosis, neurotoxicity and extracellular matrix remodeling. Nitric oxide reacts with superoxide to form peroxynitrite, which in turn nitrates tyrosine residues in proteins. Nitrotyrosine therefore serves as a marker for peroxynitrite action in a variety of disease states and in conditions of cellular damage and oxidative stress. Our OxiSelect Nitrotyrosine ELISA Kit provides a sensitive method to measure the formation of 3-nitrotyrosine in proteins.

Polystyrene-bottomed 96-well plate (ELISA)

CT361 6
EUR 73

dummy plate 10x10 cm

EVS1100-DUMMY ea
EUR 40

dummy plate 20x10 cm

EVS1200-DUMMY ea
EUR 40

dummy plate 20x20 cm

EVS1300-DUMMY ea
EUR 40

notched glass plate 10x10cm

EVS3100-NGLASS ea
EUR 48

notched glass plate 20x10cm

EVS3200-NGLASS ea
EUR 56

notched glass plate 20x20cm

EVS3300-NGLASS ea
EUR 56

Dummy plate, AE-6220

2328796 3unit
EUR 276

PCR plate-foil universal

A28216 1x100 pcs
EUR 94

PCR plate-foil universal

A28216L 5x100 pcs
EUR 310

PCR 96-Plate unskirted

A28239 25 pcs
EUR 98

Digital Hot Plate Stirrer

abx725039-1Unit 1 Unit
EUR 606

Hot Plate Stirrer Accessories

abx725045-RemoteControlPendant Remote Control Pendant
EUR 328

Hot Plate Stirrer Accessories

abx725045-SupportStandforSensorProb Support Stand for Sensor Prob
EUR 363

Hot Plate Stirrer Accessories

abx725045-TemperatureSensorProbe Temperature Sensor Probe
EUR 311

Plate Coating Buffer-CB1

KF17336 500 ml
EUR 244

Clear 384 Well Plate

X105-1EA 1EA
EUR 112

SEALING FILM, ALUMINUM, 50 µM, NONSTERILE, BULK

PCR-AS-600 100/pk
EUR 313
Description: Sealing Products; Sealing films - Axygen

Pressure Sealing Mats for 384 Well Format

GP504 5 mats
EUR 196

Protein A-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack

PRTA55-5P 1 PK
EUR 263

Protein G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack

PRTG65-5P 1 PK
EUR 263

Phenylalanine neonate (without Membrane Plate) ELISA kit

DEIA-XY11 192T
EUR 874
Description: The Phenylalanine Microwell Enzyme Assay is designed for the quantitative determination of phenylalanine in neonatal blood spots. It is for use as an aid in screening newborns for elevated phenylalanine levels.

Galactose neonatal (with Membrane Plate) ELISA kit

DEIA-XY12 192T
EUR 902
Description: The GAL-MW EA is designed for the quantitative determination of galactose in neonatal blood spots. It is for in-vitro diagnostic use as an aid in screening newborns for elevated galactose levels as seen in galactosemia.

Galactose neonatal (without Membrane Plate) ELISA kit

DEIA-XY13 192T
EUR 855
Description: The Galactose Elisa is designed for the quantitative determination of galactose in neonatal blood spots. It is for in-vitro diagnostic use as an aid in screening newborns for elevated galactose levels as seen in Galactosemia.

Phenylalanine neonate (with Membrane Plate) ELISA kit

DEIA-XY8 192T
EUR 1014
Description: The Phenylalanine Microwell Enzyme Assay is designed for the quantitative determination of phenylalanine in neonatal blood spots. It is for use as an aid in screening newborns for elevated phenylalanine levels.

AgileWasher? ELISA Plate Washer, built-in incubator

ACT-AMPW900
EUR 5599

OxiSelect Protein Carbonyl ELISA Kit (1 plate)

STA-310 96 assays
EUR 798
Description: The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species. Our OxiSelect Protein Carbonyl ELISA Kit provides a rapid, efficient method for the detection of protein carbonyl residues. The ELISA format is perfect for higher throughput and high sensitivity; we have eliminated concentration and precipitation steps allowing greater sample retention.

Protein A&G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack

PRTAG75-5P 1 PK
EUR 286

SEALING FILM ROLLER, FOR APPLYING PLATEMAX™ SEALING FILMS, WITH STURDY PLASTIC HANDLE AND SEMI-HARD ROLLER HEAD

PCR-SP-ROLLER 1/pk
EUR 60
Description: Sealing Products; Sealing films - Axygen

SEALING FILM, ALUMINUM, 35 µM, PIERCEABLE, NONSTERILE, BULK

PCR-AS-200 100/pk
EUR 357
Description: Sealing Products; Sealing films - Axygen

SEALING FILM AXYSEAL, POLYESTER, 80 µM, NONSTERILE, BULK

PCR-SP 100/pk
EUR 155
Description: Sealing Products; Sealing films - Axygen

SEALING FILM AXYSEAL, POLYESTER, 80 µM, STERILE, BULK

PCR-SP-S 100/pk
EUR 205
Description: Sealing Products; Sealing films - Axygen

SEALING TAPE,UNIVERSAL OPTICAL,NS,BK,25/100

6575 100/pk
EUR 308
Description: Genomics; Crystallography Plt & Accessories

Mixer Heated Plate Vortexer, 115V

BCM1631 1 pcs, 1 UNIT
EUR 2731.39

Microtube and PCR Plate Cooler

BCM1834 1 pcs, 1 UNIT
EUR 240.51

384 Well Plate, 10/Bag

PCR-384-SN 1PK, 10UNIT
EUR 93.5

96 WELL PCR SEGMENTED PLATE

PCR-96-SG-C 10/pk
EUR 145
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

AgileSealer? Semi Automated Plate Sealer

ACT-APS-200
EUR 4875

1 mm Plate Set, RS

2392020 2unit
EUR 258
Description: 1 pai r of gl ass pl at es + 1 gask et , for 1 mm gel

2 mm Plate Set, RS

2392024 2unit
EUR 258
Description: 1 pai r of gl ass pl at es + 1 gask et , for 2 mm gel

0.75mm Plate Set,RM-075

2393014 2unit
EUR 263
Description: 1 pai r of gl ass pl at es + 1 gask et , for 0.75 mm gel

5 mm Dummy Plate, RM

2393074 10unit
EUR 241
Description: Pl ast i c pl at e for r unni ng 1 Pagel pr ecast gel onl y

2mm Plate Set,RM-22

2393740 3unit
EUR 281
Description: 1 pai r of gl ass pl at es + 1 gask et , for 2 mm gel

Technical Observe: Utilizing enzyme-linked immunosorbent assays to judge humoral responses to vaccination in opposition to respiratory viruses in beef cattle

This experiment evaluated humoral responses in beef calves vaccinated in opposition to parainfluenza-Three virus (PI3), bovine respiratory syncytial virus (BRSV), and bovine herpesvirus-1 (BHV-1) utilizing by way of serum neutralization (SN) checks or enzyme-linked immunosorbent assays (ELISA). Blood samples have been collected from 50 overtly wholesome Angus-influenced steers (183 ± Three kg of physique weight, 212 ± 2 d of age) on d 0, 21, 35, and 49 of the experiment. Steers have been vaccinated in opposition to respiratory viruses on d Zero and 21.
Blood was processed for serum assortment and frozen in duplicates. One of many duplicates was analyzed for antibodies in opposition to BRSV, PI3, and BHV-1 utilizing commercially accessible ELISA (IDEXX Switzerland AG, Liebefeld-Bern, Switerland), and outcomes reported as pattern:constructive management (S/P, %) ratio. The opposite duplicate was analyzed for antibodies in opposition to the identical vaccine antigens by way of SN.
This methodology report outcomes as titers, the best dilution that gives full safety of the cells, which have been remodeled with base 2 log for statistical analyses. Samples have been categorized as constructive for presence of antibodies by SN if log-transformed titer ≥ 2 for all viruses, and by ELISA if S/P ratio ≥ 50% for BHV-1 or ≥ 20% for PI3 and BRSV. Day results have been detected (P < 0.01) for SN and ELISA throughout all vaccine antigens, as antibody ranges elevated after vaccine administration.
Linear suits have been detected (P < 0.01) throughout all vaccine antigens when regressing the SN and ELISA outcomes; as SN titer elevated the ELISA S/P ratio linearly elevated (P < 0.01). Kendall (τ) and Spearman’s rank (ρ) correlations have been additionally detected (P < 0.01) between SN and ELISA outcomes throughout all vaccine antigens. The SN and ELISA have been very strongly correlated (ρ ≥ 0.83) for BHV-1 and PI3, and strongly correlated (ρ = 0.66) for BRSV. Cohen’s kappa coefficient for analysis settlement between strategies have been robust for BHV-1 and PI3 (κ ≥ 0.88), however weak (κ = 0.47) for BRSV.
The sensitivity of the ELISA in yielding true constructive outcomes approached 100% throughout all antigens. The specificity of the ELISA in yielding unfavorable outcomes have been passable for BHV-1 and PI3 assays (84.Zero and 88.5%, respectively) however not for BRSV (34.4%).
Regardless of limitations in detecting true BRSV negatives, outcomes from this experiment point out that the business ELISAs examined herein can be utilized as surrogate for SN checks in quantifying humoral responses to vaccination in opposition to BHV-1, PI3, and BRSV in beef cattle.
Key phrases: beef cattle; humoral response; respiratory illness; vaccination

EZBlock? (TBS) Blocking Buffer

2117-1000
EUR 212

EZBlock? (TBS) Blocking Buffer

2117-200
EUR 125

EZBlock? (PBS) Blocking Buffer

2128-1000
EUR 229

EZBlock? (PBS) Blocking Buffer

2128-200
EUR 137

Neptune 10ul barrier tip

BT10 10 trays of 96 / pack
EUR 59
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 100ul barrier tip

BT100 10 trays of 96 / pack
EUR 59
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 1000ul barrier tip

BT1000.96 8 trays of 96 / pack
EUR 59
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 20ul barrier tip

BT20 10 trays of 96 / pack
EUR 59
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 200ul barrier tip

BT200 10 trays of 96 / pack
EUR 59
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 300ul barrier tip

BT300 10 trays of 96 / pack
EUR 59
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune Assay Diluent-AD3

KF17349 500 ml
EUR 244

Neptune Sample Diluent-SD3

KF17354 500 ml
EUR 244

Block

BSH100-02 1 PC
EUR 137.21

Block

BSH100-05 1 PC
EUR 146.78

Block

BSH100-1213 1 PC
EUR 137.21

Block

BSH100-1232 1 PC
EUR 137.21

Block

BSH100-15 1 PC
EUR 137.21

Block

BSH100-150 1 PC
EUR 156.35

Block

BSH100-1520 1 PC
EUR 137.21

Block

BSH100-15HL 1 PC
EUR 156.35

Block

BSH100-500 1 PC
EUR 104.15

Block

BSH100-5MT 1 PC
EUR 156.35

Block

BSH100-CV 1 PC
EUR 137.21

Block

BSW02 1 PC
EUR 151.93

Block

BSW05 1 PC
EUR 151.93

Block

BSW10 1 PC
EUR 151.93

Block

BSW13 1 PC
EUR 151.93

Block

BSW15 1 PC
EUR 151.93

Block

BSW1500 1 PC
EUR 151.93

Block

BSW1516 1 PC
EUR 183.03

Block

BSW1520 1 PC
EUR 151.93

Block

BSW50 1 PC
EUR 151.93

Block

BSW5MT 1 PC
EUR 151.93

Block

BSWHEM 1 PC
EUR 237.26

Block

BSWMT 1 PC
EUR 194.99

Block

BSWNMR 1 PC
EUR 481.73

Block

BSWPCR1 1 PC
EUR 255.6

Block

BSWPCR2 1 PC
EUR 208.55

Block

H5000-02 1 PC
EUR 290.33

Block

H5000-025 1 PC
EUR 591.35

Block

H5000-05 1 PC
EUR 290.33

Block

H5000-12 1 PC
EUR 382.55

Block

H5000-1232 1 PC
EUR 382.55

Block

H5000-15 1 PC
EUR 290.33

Block

H5000-150 1 PC
EUR 382.55

Block

H5000-20 1 PC
EUR 382.55

Block

H5000-500 1 PC
EUR 382.55

Block

H5000-CMB 1 PC
EUR 290.33

Block

H5000-CU 1 PC
EUR 382.55

Block

H5000-WB 1 PC
EUR 4288.85

Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed

ELISA-1 1
EUR 202

Blocking Buffer and Coating Stabilizer

abx090811-1000ml 1000 ml
EUR 523

West-Ezier Super Blocking Buffer

W3700-010 100ml
EUR 97

West-Ezier Super Blocking Buffer

W3700-050 500ml
EUR 165

West-Ezier Super Blocking Buffer

W3700-100 1L
EUR 246

PBS Blocking buffer with BSA

SB0626 1pk, 1L
EUR 83.93

TBS blocking buffer with BSA

SD8113 1pk, 1L
EUR 83.93

Permeabilization and Blocking Buffer (5X)

22017 100mL
EUR 149
Description: Minimum order quantity: 1 unit of 100mL

TrueBlack WB Blocking Buffer Kit

23013 1kit
EUR 347
Description: Minimum order quantity: 1 unit of 1kit

TrueBlack WB Blocking Buffer Kit

23013-T 1kit
EUR 125
Description: Minimum order quantity: 1 unit of 1kit

3 Blocking Buffer Sample Pack

KF17340 3 x 100 ml
EUR 205

EZBlock? T20 (TBS) Blocking Buffer

2140-1000
EUR 218

EZBlock? T20 (TBS) Blocking Buffer

2140-200
EUR 120

EZBlock? T20 (PBS) Blocking Buffer

2143-1000
EUR 218

EZBlock? T20 (PBS) Blocking Buffer

2143-200
EUR 120

Neptune 1000-1250ul barrier tip

BT1250 8 trays of 96 / pack
EUR 59
Description: Extended Length, Low retention, Racked, Pre-Sterile, Sample Saving Surface

Block-Free ELISA Reagent

M4102-250
EUR 452

Block-Free ELISA Reagent

M4102-50
EUR 147

Pro-Block

PBK-20000 20 L
EUR 1210

Pro-Block

PBK010 10 L
EUR 676

Pro-Block

PBK125 125 ml
EUR 74

Pro-Block

PBK500 500 ml
EUR 101

Pro-Block

PBK999 1000 ml
EUR 124

EZ Block

EZB125 125 ml
EUR 93

EZ Block

EZB500 500 ml
EUR 127

EZ Block

EZB999 1000 ml
EUR 173

Super Block

AAA125 125 ml
EUR 74

Super Block

AAA500 500 ml
EUR 101

Super Block

AAA999 1000 ml
EUR 124

Peroxide Block

ACA015 15 ml
EUR 67

Peroxide Block

ACA125 125 ea.
EUR 76

Peroxide Block

ACA500 500 ml
EUR 101

Peroxide Block

ACA999 1000 ml
EUR 125

ChonBlock Blocking/Sample Dilution ELISA Buffer, 100 ml

9068 100 ml
EUR 129.75
Description: ChonBlock Blocking/Sample Dilution ELISA Buffer

ELISA Plate Blocking Buffer concentrate (10X) BSA-based

80060 50 ml
EUR 202

ELISA Plate Blocking Buffer concentrate (10X) milk-based

80062 100 ml
EUR 202

Casein-based PBS Blocking Buffer Pack

AR0182 500mL/Pack
EUR 70

Casein-based TBS Blocking Buffer Pack

AR0183 500mL/Pack
EUR 70

Blocking Buffer for ICC and IHC

SF40011 10 ml
EUR 128

EZBlock? Blocking Buffer and Signal Enhancer

M4104-100
EUR 153

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