Analysis of an in-house oblique enzyme-linked immunosorbent assay of feline panleukopenia VP2 subunit antigen compared to hemagglutination inhibition assay to watch tiger antibody ranges by Bayesian strategy
Background: Feline panleukopenia virus (FPV) is an etiologic pathogen of feline panleukopenia that infects all members of Felidae together with tigers (Panthera tigris). Vaccinations in opposition to FPV amongst wild felid species have lengthy been practiced in zoos worldwide.
Nevertheless, few research have assessed the tiger immune response post-vaccination because of the absence of a serological diagnostic instrument. To deal with these limitations, this examine aimed to develop an in-home oblique enzyme-linked immunosorbent assay (ELISA) for the monitoring of tiger antibody ranges in opposition to the feline panleukopenia vaccine by using the synthesized subunit capsid protein VP2.
An in-house horseradish peroxidase (HRP) conjugated rabbit anti-tiger immunoglobulin G (IgG) polyclonal antibody (HRP-anti-tiger IgG) was produced on this examine and employed within the assay. It was then in comparison with a business HRP-conjugated goat anti-cat IgG (HRP-anti-cat IgG). Sensitivity and specificity have been evaluated utilizing the Bayesian mannequin with preferential conditional dependence between HRP-conjugated antibody-based ELISAs and hemagglutination-inhibition (HI) checks.
Outcomes: The posterior estimates for sensitivity and specificity of two oblique ELISA HRP-conjugated antibodies have been increased than these of the HI take a look at. The sensitivity and specificity of the oblique ELISA for HRP-anti-tiger IgG and HRP-anti-cat IgG have been 86.5, 57.2 and 86.7%, 64.6%, respectively, whereas the outcomes of the HI take a look at have been 79.1 and 54.1%. In purposes, 89.6% (198/221) and 89.1% (197/221) of the tiger serum samples have been decided to be seropositive by oblique ELISA testing in opposition to HRP-anti-tiger and HRP-anti-cat, respectively.
Conclusion: To one of the best of our information, the particular serology assays for the detection of the tiger IgG antibody haven’t but been established. The HRP-anti-tiger IgG has been produced for the aim of creating the particular immunoassays for tigers. Remarkably, an in-house oblique ELISA based mostly on VP2 subunit antigen has been efficiently developed on this examine, offering a probably worthwhile serological instrument for the efficient detection of tiger antibodies.
Key phrases: Bayesian mannequin; Capsid protein VP2; Feline panleukopenia virus; Hemagglutination inhibition assay; Oblique ELISA; Tiger (Panthera tigris).

Crystallization Plate Sealing Paddle Qty: 5 |
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M-CTP-PDL1 | MiTeGen | 5 PADDLES | EUR 21 |
Description: Crystallization Plate Sealing Paddle Qty: 5 |
Optical Sealing Film96 well PCR plate F |
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PR18-1X100NO | EWC Diagnostics | 1 unit | EUR 236.8 |
Description: Optical Sealing Film96 well PCR plate F |
PIERCABLE SEALING MAT FOR 24 WELL DEEP WELL PLATE |
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AM-24-SQ | CORNING | 10/pk | EUR 248.4 |
Description: Sealing Products; Sealing mats - Axygen |
PIERCABLE SEALING MAT FOR 24 WELL DEEP WELL PLATE, STERILE |
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AM-24-SQ-S | CORNING | 10/pk | EUR 280.8 |
Description: Sealing Products; Sealing mats - Axygen |
Kinesis Silicone Round Well Sealing Mat 500ul plate Slit - PK5 |
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CHR1239 | Scientific Laboratory Supplies | PK5 | EUR 76.95 |
Axygen 384 well white PCR plate for Roche 480 LightCycler no sealing - PK10 |
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AXY2090 | Scientific Laboratory Supplies | PK10 | EUR 85.05 |
Axygen 96 well white PCR plate for Roche 480 LightCycler no sealing - PK10 |
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AXY2112 | Scientific Laboratory Supplies | PK10 | EUR 67.5 |
Optically clear sealing film for qPCR, Peelable, for most plate types, 100/pk |
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MS1000-PCR1 | Benchmark Scientific | 1 each | EUR 149.35 |
Optically clear sealing film for qPCR, Pierceable, for most plate types, 100/pk |
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MS1000-PCR3 | Benchmark Scientific | 1 each | EUR 149.35 |
IntelliXseal SA Weighted Sealing Platen - EA |
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59-2008 | Scientific Laboratory Supplies | EA | EUR 102.6 |
Axygen 384 well white PCR plate for Roche 480 LightCycler with UC-500 sealing - PK10 |
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AXY2086 | Scientific Laboratory Supplies | PK10 | EUR 149.85 |
SEALING FILM ROLLER, FOR APPLYING PLATEMAX™ SEALING FILMS, WITH STURDY PLASTIC HANDLE AND SEMI-HARD ROLLER HEAD |
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PCR-SP-ROLLER | CORNING | 1/pk | EUR 72 |
Description: Sealing Products; Sealing films - Axygen |
AgileSealerTM Semi Automated Plate Sealer (including 1 adaptor for 96-well PCR plate, 1 adaptor for flexible plates, and 1 weighted sealing flake) |
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APS-200 | Avans Biotechnology Corp | each | EUR 2800 |
Sealing Film For Plates PP/PE With Adhesive - PK50 |
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701370 | Scientific Laboratory Supplies | PK50 | EUR 307.8 |
Axygen 96 well bar-coded white PCR plate for Roche 480 LightCycler with UC-500 sealing - PK50 |
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AXY2110 | Scientific Laboratory Supplies | PK50 | EUR 623.7 |
Kinesis Silicone Round Well Sealing Mat 1.1ml Plates; Slit - PK5 |
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CHR1237 | Scientific Laboratory Supplies | PK5 | EUR 64.8 |
PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500 |
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HS-150-QPCR | CORNING | 100/pk | EUR 381.6 |
Description: Sealing Products; Sealing films - Axygen |
PLATEMAX PEELABLE HEAT SEALING FILM FOR COMPOUND STORAGE. 100/500 |
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HS-400 | CORNING | 100/pk | EUR 364.8 |
Description: Sealing Products; Sealing films - Axygen |
PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500 |
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HS-100-QPCR | CORNING | 100/pk | EUR 381.6 |
Description: Sealing Products; Sealing films - Axygen |
Crystallization Plate Support Clear acrylic support block that fits under InSitu, CD1, CD2 plates. Primary use to support plate center when applying sealing film. |
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M-CTP-PSS-1 | MiTeGen | 1 Unit | EUR 39 |
Description: Crystallization Plate SupportClear acrylic support block that fits under InSitu, CD1, CD2 plates. Primary use to support plate center when applying sealing film. |
Optically clear sealing film for qPCR, Strong Bond, for PP plates, 100/pk |
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MS1000-PCR2 | Benchmark Scientific | 1 each | EUR 149.35 |
UVXPO Sealing Sheets |
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CTP-106 | Jena Bioscience GmbH | 100pcs. | EUR 323.38 |
Sealing plugs GE - PK5 |
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19003901 | Scientific Laboratory Supplies | PK5 | EUR 85.05 |
PLATEMAX PIERCEABLE ALUMINUM HEAT SEALING FILM FOR STORAGE APPLICATIONS. 100/500 |
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HS-300 | CORNING | 100/pk | EUR 364.8 |
Description: Sealing Products; Sealing films - Axygen |
TAPE-SEQUENCING SEALING |
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IB81630 | IBI Scientific | - | EUR 60.28 |
SafeStore Silicone Sealing Mat for 96 Round Well 1.0ml plates, piercable - 50 mats |
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WB0566 | Westburg | each | EUR 296.48 |
SafeStore Silicone Sealing Mat for 96 Round Well 2.0ml plates, piercable - 50 mats |
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WB0567 | Westburg | each | EUR 296.48 |
POLYPROPYLENE SEALING FILM |
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PR21-1X100NO | EWC Diagnostics | 1 unit | EUR 133.88 |
Description: POLYPROPYLENE SEALING FILM |
Sealing Film, 50 Sheets |
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TB418 | Bio Basic | 1PK, 50UNIT | EUR 73.57 |
Sealing Ring Svl15 - PK10 |
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701-01 | Scientific Laboratory Supplies | PK10 | EUR 76.95 |
Axymat Silicone Sealing Mat |
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CCM-103 | Jena Bioscience GmbH | 10pcs. | EUR 95.84 |
SEALING FILM, PARAFILM, 2x250 |
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S801 | PhytoTechnology Laboratories | 12EA | EUR 51.54 |
SEALING FILM, PARAFILM; 4x125 |
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S823 | PhytoTechnology Laboratories | 12EA | EUR 34.66 |
SEALING FILM, PARAFILM, 4x250 |
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S911 | PhytoTechnology Laboratories | 10EA | EUR 71.79 |
Sealing Film Polyester - PK100 |
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MOL2374 | Scientific Laboratory Supplies | PK100 | EUR 318.6 |
PLATEMAX PEELABLE HEAT SEALING FILM FOR LOW TEMPERATURE COMPOUND STORAGE AND PCR. 100/500 |
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HS-200 | CORNING | 100/pk | EUR 364.8 |
Description: Sealing Products; Sealing films - Axygen |
Sealing Film Polypropylene - PK100 |
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MOL2372 | Scientific Laboratory Supplies | PK100 | EUR 229.5 |
Clear Heat Sealing Seals (100sheets) |
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MAAB-0812 | Westburg | each | EUR 190.75 |
Sealing Film, Sterile, 50 Sheets |
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TB418-S | Bio Basic | 1PK, 50UNIT | EUR 74.62 |
Eppendorf Heat Sealing Film - EACH |
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E0030127838 | Scientific Laboratory Supplies | EACH | EUR 232.2 |
Sealing Film, Foil Tight Seal, 100Sheet |
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SMT4406 | Bio Basic | 1PK, 100UNIT | EUR 247.92 |
Eppendorf Heat Sealing Foil - PK100 |
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E0030127854 | Scientific Laboratory Supplies | PK100 | EUR 164.7 |
Sealing film for Automation - PK100 |
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MOL2258 | Scientific Laboratory Supplies | PK100 | EUR 225.45 |
Nunc Sealing Tape Polyolefin - PK100 |
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235307 | Scientific Laboratory Supplies | PK100 | EUR 777.6 |
DURAN Silicone Sealing Gasket - PK10 |
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BOT2280 | Scientific Laboratory Supplies | PK10 | EUR 51.3 |
SEALING MAT, FOR 1.1 AND 2 ML 96 WELL DEEP WELL PLATES, ROUND WELLS, SILICONE, NATURAL, NONSTERILE, BULK |
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AM-2ML-RD | CORNING | 10/pk | EUR 196.8 |
Description: Sealing Products; Sealing mats - Axygen |
Sealing Cap Nalgene (250/290Ml) - PK2 |
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CEN9034 | Scientific Laboratory Supplies | PK2 | EUR 143.1 |
Sealing Caps For Cen9526/36 - PK2 |
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CEN9544 | Scientific Laboratory Supplies | PK2 | EUR 48.6 |
Sealing Mats for 96 Well Format |
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GP501 | GenDepot | 5 mats | EUR 176.4 |
Sealing Mat for DWP 96/2000 - PK50 |
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E0030127960 | Scientific Laboratory Supplies | PK50 | EUR 199.8 |
96 Well MPlate Sealing Strip - PK24 |
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CHR5886 | Scientific Laboratory Supplies | PK24 | EUR 79.65 |
Heat Seals ALPS Microplate Sealing - PK100 |
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AB0559 | Scientific Laboratory Supplies | PK100 | EUR 168.75 |
Replacement sealing ring, (GL45), 10/pk. |
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B3000-RIN | Benchmark Scientific | 1 PC | EUR 14.94 |
Replacement sealing ring, (GL32), 10/pk. |
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B3000-RIN2 | Benchmark Scientific | 1 PC | EUR 14.94 |
Hettich Sealing lid 250ml Bucket - EACH |
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CEN0717 | Scientific Laboratory Supplies | EACH | EUR 190.35 |
Sealing film, Aluminum membrane, pk/100 |
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P1001-A | MTC Bio | 100/pack | EUR 82.68 |
Description: Sealing film Aluminum membrane |
Boot Covers - PK200 |
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SAF0340 | Scientific Laboratory Supplies | PK200 | EUR 525.15 |
Sealing Mat (Round Cap Pierceable) - PK50 |
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AB-0566 | Scientific Laboratory Supplies | PK50 | EUR 282.15 |
Peel-seal Heat Sealing Sheets - PK100 |
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PCR0580 | Scientific Laboratory Supplies | PK100 | EUR 122.85 |
Sealing Mats round cap autoclavable (50) |
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AB-0674 | Westburg | each | EUR 162.41 |
Kinesis Sealing Ring; 1/16; PTFE - PK20 |
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CHR1847 | Scientific Laboratory Supplies | PK20 | EUR 72.9 |
Easy Pierce 20µm Heat Sealing Foil (100) |
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MAAB-1720 | Westburg | each | EUR 117.18 |
Nunc Sealing Tape 134x80mm Sterile - PK200 |
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236366K | Scientific Laboratory Supplies | PK200 | EUR 156.6 |
Sealing Mats square cap autoclavable (50) |
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AB-0675 | Westburg | each | EUR 162.41 |
Axygen Aluminium sealing film 35um - PK500 |
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AXY2132 | Scientific Laboratory Supplies | PK500 | EUR 808.65 |
Axygen Aluminium sealing film 50um - PK500 |
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AXY2250 | Scientific Laboratory Supplies | PK500 | EUR 703.35 |
sealing Agent, 50 mL/vial, 4 vials/cs |
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208221 | NEST Biotechnology | 40 pcs/cs | EUR 160.49 |
Description: sealing Agent, 50 mL/vial, 4 vials/cs |
sealing Agent, 50 mL/vial, 4 vials/cs |
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211121 | NEST Biotechnology | 4 pcs/cs | EUR 149.82 |
Description: sealing Agent, 50 mL/vial, 4 vials/cs |
Kinesis Sealing Ring Graphite Thermo - PK10 |
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CHR1816 | Scientific Laboratory Supplies | PK10 | EUR 116.1 |
Sealing Caps For Cen9522/24/32/34 - PK2 |
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CEN9542 | Scientific Laboratory Supplies | PK2 | EUR 40.5 |
Sealing Mat for DWP 96/1000 or /500 - PK50 |
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E0030127978 | Scientific Laboratory Supplies | PK50 | EUR 199.8 |
Nunc polyolifin Sealing Tape 121x79mm - PK100 |
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232701 | Scientific Laboratory Supplies | PK100 | EUR 256.5 |
SEALING TAPE APPLICATOR MANUAL; 2/PK - PK2 |
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60180-M950 | Scientific Laboratory Supplies | PK2 | EUR 37.8 |
Hettich Sealing lid for 250ml bucket - EACH |
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CEN0622 | Scientific Laboratory Supplies | EACH | EUR 153.9 |
Pressure Sealing Mats for 384 Well Format |
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GP504 | GenDepot | 5 mats | EUR 235.2 |
PCR Sealing Film, 146 × 81 mm, 100/pk, 500/cs |
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410001 | NEST Biotechnology | 500 pcs/cs | EUR 127.38 |
Description: PCR Sealing Film, 146 × 81 mm, 100/pk, 500/cs |
PCR Sealing Film, 141 × 78 mm, 100/pk, 500/cs |
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410011 | NEST Biotechnology | 500 pcs/cs | EUR 669.24 |
Description: PCR Sealing Film, 141 × 78 mm, 100/pk, 500/cs |
Technical Observe: Utilizing enzyme-linked immunosorbent assays to judge humoral responses to vaccination in opposition to respiratory viruses in beef cattle
This experiment evaluated humoral responses in beef calves vaccinated in opposition to parainfluenza-Three virus (PI3), bovine respiratory syncytial virus (BRSV), and bovine herpesvirus-1 (BHV-1) utilizing by way of serum neutralization (SN) checks or enzyme-linked immunosorbent assays (ELISA). Blood samples have been collected from 50 overtly wholesome Angus-influenced steers (183 ± Three kg of physique weight, 212 ± 2 d of age) on d 0, 21, 35, and 49 of the experiment. Steers have been vaccinated in opposition to respiratory viruses on d Zero and 21.
Blood was processed for serum assortment and frozen in duplicates. One of many duplicates was analyzed for antibodies in opposition to BRSV, PI3, and BHV-1 utilizing commercially accessible ELISA (IDEXX Switzerland AG, Liebefeld-Bern, Switerland), and outcomes reported as pattern:constructive management (S/P, %) ratio. The opposite duplicate was analyzed for antibodies in opposition to the identical vaccine antigens by way of SN.
This methodology report outcomes as titers, the best dilution that gives full safety of the cells, which have been remodeled with base 2 log for statistical analyses. Samples have been categorized as constructive for presence of antibodies by SN if log-transformed titer ≥ 2 for all viruses, and by ELISA if S/P ratio ≥ 50% for BHV-1 or ≥ 20% for PI3 and BRSV. Day results have been detected (P < 0.01) for SN and ELISA throughout all vaccine antigens, as antibody ranges elevated after vaccine administration.
Linear suits have been detected (P < 0.01) throughout all vaccine antigens when regressing the SN and ELISA outcomes; as SN titer elevated the ELISA S/P ratio linearly elevated (P < 0.01). Kendall (τ) and Spearman’s rank (ρ) correlations have been additionally detected (P < 0.01) between SN and ELISA outcomes throughout all vaccine antigens. The SN and ELISA have been very strongly correlated (ρ ≥ 0.83) for BHV-1 and PI3, and strongly correlated (ρ = 0.66) for BRSV. Cohen’s kappa coefficient for analysis settlement between strategies have been robust for BHV-1 and PI3 (κ ≥ 0.88), however weak (κ = 0.47) for BRSV.
The sensitivity of the ELISA in yielding true constructive outcomes approached 100% throughout all antigens. The specificity of the ELISA in yielding unfavorable outcomes have been passable for BHV-1 and PI3 assays (84.Zero and 88.5%, respectively) however not for BRSV (34.4%).
Regardless of limitations in detecting true BRSV negatives, outcomes from this experiment point out that the business ELISAs examined herein can be utilized as surrogate for SN checks in quantifying humoral responses to vaccination in opposition to BHV-1, PI3, and BRSV in beef cattle.
Key phrases: beef cattle; humoral response; respiratory illness; vaccination
Alternative Block ELISA Blocking Buffer, Synthetic |
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MBS258395-500mL | MyBiosource | 500mL | EUR 230 |
Alternative Block ELISA Blocking Buffer, Synthetic |
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MBS258395-5x1L | MyBiosource | 5x1L | EUR 1435 |
Monster Block ELISA Blocking Buffer, Non-Mammalian |
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MBS258394-100mL | MyBiosource | 100mL | EUR 160 |
Monster Block ELISA Blocking Buffer, Non-Mammalian |
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MBS258394-1L | MyBiosource | 1L | EUR 345 |
Monster Block ELISA Blocking Buffer, Non-Mammalian |
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MBS258394-500mL | MyBiosource | 500mL | EUR 230 |
Monster Block ELISA Blocking Buffer, Non-Mammalian |
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MBS258394-5x1L | MyBiosource | 5x1L | EUR 1435 |
Alternative Block™ ELISA Blocking Buffer, Synthetic |
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6299 | Immunochemistry | 100 mL | EUR 52.25 |
Alternative Block™ ELISA Blocking Buffer, Synthetic |
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6300 | Immunochemistry | 500 mL | EUR 145 |
Alternative Block™ ELISA Blocking Buffer, Synthetic |
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6301 | Immunochemistry | 1 L | EUR 290 |
Monster Block™ ELISA Blocking Buffer, Non-Mammalian |
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6295 | Immunochemistry | 100 mL | EUR 52.25 |
Monster Block™ ELISA Blocking Buffer, Non-Mammalian |
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6296 | Immunochemistry | 500 mL | EUR 145 |
Monster Block™ ELISA Blocking Buffer, Non-Mammalian |
|||
6297 | Immunochemistry | 1 L | EUR 290 |
OOSA11206-1L - ELISA NEPTUNE BUFFER Accessory Reagent |
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OOSA11206-1L | Aviva Systems Biology | 1000ml | EUR 749 |
OOSA09562-100ML - ELISA NEPTUNE BUFFER Accessory Reagent |
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OOSA09562-100ML | Aviva Systems Biology | 100ml | EUR 169 |
OOSA11205-500ML - ELISA NEPTUNE BUFFER Accessory Reagent |
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OOSA11205-500ML | Aviva Systems Biology | 500ml | EUR 519 |
Blocking Buffer for ELISA |
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abx090807-500ml | Abbexa | 500 ml | EUR 460.8 |
Blocking Buffer for ELISA |
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GWB-E120C6 | GenWay Biotech | 500 ml | Ask for price |
Blocking Buffer for ELISA |
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MBS318404-500mL | MyBiosource | 500mL | EUR 335 |
Blocking Buffer for ELISA |
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MBS318404-5x500mL | MyBiosource | 5x500mL | EUR 1315 |
ELISA Plate Blocking Buffer |
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E-ELIR-003-24Tests | Elabscience Biotech | 24 Tests | Ask for price |
ELISA Plate Blocking Buffer |
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E-ELIR-003-48Tests | Elabscience Biotech | 48 Tests | EUR 110 |
ELISA Plate Blocking Buffer |
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E-ELIR-003-96Tests | Elabscience Biotech | 96 Tests | EUR 35 |
ELISA Plate Blocking Buffer |
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E-ELIR-003-96Tests10 | Elabscience Biotech | 96 Tests *10 | EUR 350 |
ELISA Plate Blocking Buffer |
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E-ELIR-003-96Tests5 | Elabscience Biotech | 96 Tests *5 | EUR 175 |
ELISA Plate Blocking Buffer |
|||
MBS2571969-100mL | MyBiosource | 100mL | EUR 110 |
ELISA Plate Blocking Buffer |
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MBS2571969-1L | MyBiosource | 1L | EUR 165 |
PanBlock ELISA Blocking Buffer |
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BUF81201-100 | The Native Antigen Company | each | EUR 81.12 |
Description: Blocking Buffer for antigen-down and sandwich ELISA. PanBlock is for antigen-down and sandwich ELISAs and for those with high background problems or cross-react with mammalian buffers. |
PanBlock ELISA Blocking Buffer |
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BUF81201-1000 | The Native Antigen Company | each | EUR 384.98 |
Description: Blocking Buffer for antigen-down and sandwich ELISA. PanBlock is for antigen-down and sandwich ELISAs and for those with high background problems or cross-react with mammalian buffers. |
SynBlock ELISA Blocking Buffer |
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MBS258400-100mL | MyBiosource | 100mL | EUR 160 |
SynBlock ELISA Blocking Buffer |
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MBS258400-1L | MyBiosource | 1L | EUR 345 |
SynBlock ELISA Blocking Buffer |
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MBS258400-500mL | MyBiosource | 500mL | EUR 245 |
SynBlock ELISA Blocking Buffer |
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MBS258400-5x1L | MyBiosource | 5x1L | EUR 1435 |
Blocking Buffer |
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abx098972-100l | Abbexa | 100 µl | EUR 93.75 |
Blocking Buffer |
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abx098972-1ml | Abbexa | 1 ml | Ask for price |
Blocking Buffer |
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abx098972-1vial | Abbexa | 1 vial | EUR 184.8 |
Blocking Buffer |
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abx098972-200l | Abbexa | 200 µl | Ask for price |
Blocking Buffer |
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CBB101 | Signosis | 60 ml | EUR 87 |
Blocking buffer |
|||
IBS-BB005 | iNtRON Biotechnology Inc | 500 mL | EUR 49 |
Blocking Buffer |
|||
ML044-100ML | EWC Diagnostics | 1 unit | EUR 4.75 |
Description: Blocking Buffer |
Blocking Buffer |
|||
ML044-500ML | EWC Diagnostics | 1 unit | EUR 16.62 |
Description: Blocking Buffer |
Blocking Buffer |
|||
RM01757 | Abclonal | 20mL | EUR 9.21 |
SynBlock™ ELISA Blocking Buffer |
|||
642 | Immunochemistry | 500 mL | EUR 159.25 |
SynBlock™ ELISA Blocking Buffer |
|||
643 | Immunochemistry | 1 L | EUR 290.5 |
Synthetic Blocking Buffer-for ELISA |
|||
4520A | Kementec Solutions | 1 L | EUR 150 |
Synthetic Blocking Buffer-for ELISA |
|||
4520L | Kementec Solutions | 500 ML | EUR 111 |
5% Blocking Buffer |
|||
E-IR-R107-10mL | Elabscience Biotech | 10mL | EUR 10 |
5% Blocking Buffer |
|||
E-IR-R107-50mL | Elabscience Biotech | 50mL | EUR 30 |
5% Blocking Buffer |
|||
E-IR-R107-5mL | Elabscience Biotech | 5mL | EUR 8 |
5% Blocking Buffer |
|||
E-IR-R107-each | Elabscience Biotech | each | Ask for price |
Western blocking buffer |
|||
IBS-BW005 | iNtRON Biotechnology Inc | 100 mL | EUR 24.5 |
Western blocking buffer |
|||
IBS-BW005a | iNtRON Biotechnology Inc | 500 mL | EUR 70 |
Western Blocking Buffer |
|||
EZWB04-4-100mL | Shanghai WSHT Biotechnology | 100mL | EUR 10.8 |
Western Blocking Buffer |
|||
EZWB04-4-500mL | Shanghai WSHT Biotechnology | 500mL | EUR 38.4 |
Western Blocking Buffer |
|||
MBS355690-100mL | MyBiosource | 100mL | EUR 180 |
Western Blocking Buffer |
|||
MBS355690-5x100mL | MyBiosource | 5x100mL | EUR 500 |
OOMA00026-500ML - Blocking Buffer for ELISA |
|||
OOMA00026-500ML | Aviva Systems Biology | 500ML | EUR 349 |
General Blocker Blocking Buffer |
|||
632 | Immunochemistry | 100 mL | EUR 45.6 |
General Blocker Blocking Buffer |
|||
640 | Immunochemistry | 1 L | EUR 260.5 |
General Blocker Blocking Buffer |
|||
MBS258397-100mL | MyBiosource | 100mL | EUR 150 |
General Blocker Blocking Buffer |
|||
MBS258397-1L | MyBiosource | 1L | EUR 320 |
General Blocker Blocking Buffer |
|||
MBS258397-500mL | MyBiosource | 500mL | EUR 230 |
General Blocker Blocking Buffer |
|||
MBS258397-5x1L | MyBiosource | 5x1L | EUR 1325 |
Blocking Buffer for WB |
|||
GWB-41822F | GenWay Biotech | 500 ml | Ask for price |
5% BSA Blocking Buffer |
|||
MBS2567133-10mL | MyBiosource | 10mL | EUR 80 |
5% BSA Blocking Buffer |
|||
MBS2567133-50mL | MyBiosource | 50mL | EUR 95 |
5% BSA Blocking Buffer |
|||
MBS2567133-5mL | MyBiosource | 5mL | EUR 80 |
5% BSA Blocking Buffer |
|||
MBS2567133-5x50mL | MyBiosource | 5x50mL | EUR 390 |
T-Pro Blocking Buffer |
|||
JK92-W005 | T-Pro Biotechnology | 500ml/BT | EUR 60 |
TBS Blocking Buffer Pack |
|||
AR0143 | BosterBio | 200mL/pack | EUR 78 |
Blocking Buffer, 10X, 250ML |
|||
X109-250ML | Arbor Assays | 250ML | EUR 360 |
Blocking Buffer, 10X, 25ML |
|||
X109-25ML | Arbor Assays | 25ML | EUR 59 |
10X Casein Blocking Buffer |
|||
IBS-BC052 | iNtRON Biotechnology Inc | 1L | EUR 105 |
EZBlock? (PBS) Blocking Buffer |
|||
2128-1000 | Biovision | each | EUR 274.8 |
EZBlock? (PBS) Blocking Buffer |
|||
2128-200 | Biovision | each | EUR 164.4 |
EZBlock? (TBS) Blocking Buffer |
|||
2117-1000 | Biovision | each | EUR 254.4 |
EZBlock? (TBS) Blocking Buffer |
|||
2117-200 | Biovision | each | EUR 150 |
ELISA Plate Blocking Buffer concentrate (10X) BSA-based |
|||
80060 | Alpha Diagnostics | 50 ml | EUR 242.4 |
General Blocking Buffer-BB1 |
|||
KF17337 | Neuromics | 500 ml | EUR 277.2 |
SynBlock Blocking Buffer-BB3 |
|||
KF17339 | Neuromics | 500 ml | EUR 292.8 |
OORA01662-1U - Blocking Buffer |
|||
OORA01662-1U | Aviva Systems Biology | 1Each | EUR 630 |
PBS Blocking buffer with BSA |
|||
SB0626 | Bio Basic | 1pk, 1L | EUR 100.72 |
TBS blocking buffer with BSA |
|||
SD8113 | Bio Basic | 1pk, 1L | EUR 100.72 |
OORA01662-1EA - Blocking Buffer |
|||
OORA01662-1EA | Aviva Systems Biology | 1Each | EUR 569 |
OORA01673-10U - Blocking Buffer |
|||
OORA01673-10U | Aviva Systems Biology | 1Each | EUR 1735 |
OORA01673-1EA - Blocking Buffer |
|||
OORA01673-1EA | Aviva Systems Biology | 1Each | EUR 1569 |
OORA01674-10U - Blocking Buffer |
|||
OORA01674-10U | Aviva Systems Biology | 1Each | EUR 2375 |
OORA01674-1EA - Blocking Buffer |
|||
OORA01674-1EA | Aviva Systems Biology | 1Each | EUR 2139 |
Casein Blocking Buffer in PBS |
|||
40320020-1 | Bio-WORLD | 500 mL | EUR 54.82 |
Casein Blocking Buffer in PBS |
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40320020-2 | Bio-WORLD | 1 L | EUR 86.22 |
Casein Blocking Buffer in TBS |
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40320028-1 | Bio-WORLD | 500 mL | EUR 52.79 |
Casein Blocking Buffer in TBS |
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40320028-2 | Bio-WORLD | 1 L | EUR 86.19 |
OORA01675-50ML - Blocking Buffer |
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OORA01675-50ML | Aviva Systems Biology | 50mL | EUR 79 |
10× Normal Goat Blocking Buffer |
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E-IR-R111-10mL | Elabscience Biotech | 10mL | EUR 20 |
10× Normal Goat Blocking Buffer |
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E-IR-R111-5mL | Elabscience Biotech | 5mL | EUR 15 |
10× Normal Goat Blocking Buffer |
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E-IR-R111-each | Elabscience Biotech | each | Ask for price |
3 Blocking Buffer Sample Pack |
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KF17340 | Neuromics | 3 x 100 ml | EUR 246 |
OORA01665-500ML - Blocking Buffer |
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OORA01665-500ML | Aviva Systems Biology | 500mL | EUR 229 |
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