Analysis of an in-house oblique enzyme-linked immunosorbent assay of feline panleukopenia VP2 subunit antigen compared to hemagglutination inhibition assay to watch tiger antibody ranges by Bayesian strategy
Background: Feline panleukopenia virus (FPV) is an etiologic pathogen of feline panleukopenia that infects all members of Felidae together with tigers (Panthera tigris). Vaccinations in opposition to FPV amongst wild felid species have lengthy been practiced in zoos worldwide.
Nevertheless, few research have assessed the tiger immune response post-vaccination because of the absence of a serological diagnostic instrument. To deal with these limitations, this examine aimed to develop an in-home oblique enzyme-linked immunosorbent assay (ELISA) for the monitoring of tiger antibody ranges in opposition to the feline panleukopenia vaccine by using the synthesized subunit capsid protein VP2.
An in-house horseradish peroxidase (HRP) conjugated rabbit anti-tiger immunoglobulin G (IgG) polyclonal antibody (HRP-anti-tiger IgG) was produced on this examine and employed within the assay. It was then in comparison with a business HRP-conjugated goat anti-cat IgG (HRP-anti-cat IgG). Sensitivity and specificity have been evaluated utilizing the Bayesian mannequin with preferential conditional dependence between HRP-conjugated antibody-based ELISAs and hemagglutination-inhibition (HI) checks.
Outcomes: The posterior estimates for sensitivity and specificity of two oblique ELISA HRP-conjugated antibodies have been increased than these of the HI take a look at. The sensitivity and specificity of the oblique ELISA for HRP-anti-tiger IgG and HRP-anti-cat IgG have been 86.5, 57.2 and 86.7%, 64.6%, respectively, whereas the outcomes of the HI take a look at have been 79.1 and 54.1%. In purposes, 89.6% (198/221) and 89.1% (197/221) of the tiger serum samples have been decided to be seropositive by oblique ELISA testing in opposition to HRP-anti-tiger and HRP-anti-cat, respectively.
Conclusion: To one of the best of our information, the particular serology assays for the detection of the tiger IgG antibody haven’t but been established. The HRP-anti-tiger IgG has been produced for the aim of creating the particular immunoassays for tigers. Remarkably, an in-house oblique ELISA based mostly on VP2 subunit antigen has been efficiently developed on this examine, offering a probably worthwhile serological instrument for the efficient detection of tiger antibodies.
Key phrases: Bayesian mannequin; Capsid protein VP2; Feline panleukopenia virus; Hemagglutination inhibition assay; Oblique ELISA; Tiger (Panthera tigris).

A8 shoe covers SM |
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SAF4762 | Scientific Laboratory Supplies | PK300 | EUR 167.58 |
A8 shoe covers Univ |
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SAF4764 | Scientific Laboratory Supplies | PK300 | EUR 156.18 |
A8 shoe covers XL |
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SAF4766 | Scientific Laboratory Supplies | PK300 | EUR 167.58 |
Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed |
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ELISA-1 | Alpha Diagnostics | 1 | EUR 242.4 |
PIERCABLE SEALING MAT FOR 24 WELL DEEP WELL PLATE, STERILE |
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AM-24-SQ-S | CORNING | 10/pk | EUR 280.8 |
Description: Sealing Products; Sealing mats - Axygen |
SCOTT extra couch covers blue |
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CLE7044 | Scientific Laboratory Supplies | 12ROLL | EUR 377.34 |
Overshoes 16 inch Blue Covers |
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SAF0264 | Scientific Laboratory Supplies | PK100 | EUR 23.37 |
FiveGo Electrode clip and covers |
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PHM0034 | Scientific Laboratory Supplies | EACH | EUR 20.29 |
Axygen 384 well white PCR plate for Roche 480 LightCycler no sealing |
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AXY2090 | Scientific Laboratory Supplies | PK10 | EUR 69.54 |
Axygen 96 well white PCR plate for Roche 480 LightCycler no sealing |
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AXY2112 | Scientific Laboratory Supplies | PK10 | EUR 54.49 |
8 PCR Tubes With Flat Covers |
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781316 | Scientific Laboratory Supplies | EACH | EUR 204.63 |
BM Series Display covers 5 pcs |
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BAL4657 | Scientific Laboratory Supplies | EACH | EUR 55.86 |
Replacement sealing ring |
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B3000-RIN | Benchmark Scientific | 1 PC | EUR 88.52 |
Replacement sealing ring |
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B3000-RIN2 | Benchmark Scientific | 1 PC | EUR 88.52 |
Sealing Ring Svl15 |
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701-01 | Scientific Laboratory Supplies | PK10 | EUR 58.62 |
Sealing plugs GE |
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19003901 | Scientific Laboratory Supplies | PK5 | EUR 64.98 |
Sealing Film Polypropylene |
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MOL2372 | Scientific Laboratory Supplies | PK100 | EUR 170.54 |
Sealing Film Polyester |
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MOL2374 | Scientific Laboratory Supplies | PK100 | EUR 236.09 |
Optically clear sealing film for qPCR, Peelable, for most plate types, 100/pk |
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MS1000-PCR1 | Benchmark Scientific | 1 each | EUR 145 |
Optically clear sealing film for qPCR, Pierceable, for most plate types, 100/pk |
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MS1000-PCR3 | Benchmark Scientific | 1 each | EUR 145 |
Full-size Injector Rack Covers for 1600LXP |
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WAS2034 | Scientific Laboratory Supplies | EACH | EUR 524.4 |
Axygen 384 well white PCR plate for Roche 480 LightCycler with UC-500 sealing |
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AXY2086 | Scientific Laboratory Supplies | PK10 | EUR 121.98 |
Sealing Film, 50 Sheets |
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TB418 | Bio Basic | 1PK, 50UNIT | EUR 73.57 |
Nunc Sealing Tape Polyolefin |
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235307 | Scientific Laboratory Supplies | PK100 | EUR 633.84 |
Sealing film for Automation |
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MOL2258 | Scientific Laboratory Supplies | PK100 | EUR 169.4 |
Eppendorf Heat Sealing Film |
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E0030127838 | Scientific Laboratory Supplies | EACH | EUR 184.68 |
Eppendorf Heat Sealing Foil |
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E0030127854 | Scientific Laboratory Supplies | PK100 | EUR 129.96 |
DURAN Silicone Sealing Gasket |
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BOT2280 | Scientific Laboratory Supplies | PK10 | EUR 38.76 |
Dummy plate 1 plate |
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2393037 | Atto | 8unit | EUR 345.6 |
Description: For M i ni Sl ab Gel Cast |
Space plate 1 plate |
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2393038 | Atto | 15unit | EUR 331.2 |
Description: For M i ni Sl ab Gel Cast |
Sealing Film, Sterile, 50 Sheets |
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TB418-S | Bio Basic | 1PK, 50UNIT | EUR 74.62 |
Corning Breathable Sealing Tape Sterile |
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3345 | Scientific Laboratory Supplies | PK500 | EUR 638.4 |
IntelliXseal SA Weighted Sealing Platen |
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59-2008 | Scientific Laboratory Supplies | EA | EUR 55.97 |
Sealing Mat (Round Cap Pierceable) |
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AB-0566 | Scientific Laboratory Supplies | PK50 | EUR 224.58 |
Heat Seals ALPS Microplate Sealing |
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AB0559 | Scientific Laboratory Supplies | PK100 | EUR 116.28 |
Axygen Aluminium sealing film 35um |
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AXY2132 | Scientific Laboratory Supplies | PK500 | EUR 631.56 |
Nunc polyolifin Sealing Tape 121x79mm |
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232701 | Scientific Laboratory Supplies | PK100 | EUR 208.62 |
Nunc Sealing Tape 134x80mm Sterile |
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236366K | Scientific Laboratory Supplies | PK200 | EUR 127.68 |
Axygen Aluminium sealing film 50um |
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AXY2250 | Scientific Laboratory Supplies | PK500 | EUR 549.48 |
Benchmark Replacement Sealing Ring 50mL |
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BOT1911 | Scientific Laboratory Supplies | PK10 | EUR 16.63 |
Kinesis Sealing Ring Graphite Thermo |
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CHR1816 | Scientific Laboratory Supplies | PK10 | EUR 75.24 |
96 Well MPlate Sealing Strip |
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CHR5886 | Scientific Laboratory Supplies | PK24 | EUR 52.44 |
Peel-seal Heat Sealing Sheets |
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PCR0580 | Scientific Laboratory Supplies | PK100 | EUR 66.12 |
Hettich Sealing lid 250ml Bucket |
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CEN0717 | Scientific Laboratory Supplies | EACH | EUR 124.26 |
Sealing Cap Nalgene (250/290Ml) |
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CEN9034 | Scientific Laboratory Supplies | PK2 | EUR 111.72 |
Sealing Caps For Cen9526/36 |
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CEN9544 | Scientific Laboratory Supplies | PK2 | EUR 37.62 |
Axygen 96 well bar-coded white PCR plate for Roche 480 LightCycler with UC-500 sealing |
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AXY2110 | Scientific Laboratory Supplies | PK50 | EUR 510.72 |
Multiple tumor tissue array covers 53 types of benign |
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BCN1021 | TissueArray | each | EUR 266 |
Description: Multiple tumor tissue array covers 53 types of benign, malignant, regional lymph node and distant metastasis tumors with normal tissues as controls, including pathology grade, TNM/Stage (AJCC 8th edition), 102 cases/102 cores (core size 1.5mm) |
Crystallization Plate Support Clear acrylic support block that fits under InSitu, CD1, CD2 plates. Primary use to support plate center when applying sealing film. |
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M-CTP-PSS-1 | MiTeGen | 1 Unit | EUR 39 |
Description: Crystallization Plate SupportClear acrylic support block that fits under InSitu, CD1, CD2 plates. Primary use to support plate center when applying sealing film. |
Plate Carrier for plate/tube bucket |
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E5920705008 | Scientific Laboratory Supplies | EACH | EUR 281.58 |
INTELLI-PLATE 48-well optimization plate |
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MAR-102-0002-00 | MiTeGen | 120 PLATES | EUR 990 |
Description: INTELLI-PLATE 48-well optimization plate; 120 plates |
INTELLI-PLATE 48-well screening plate |
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MAR-102-0003-00 | MiTeGen | 120 PLATES | EUR 990 |
Description: INTELLI-PLATE 48-well screening plate; 120 plates |
Sealing film, Aluminum membrane, pk/100 |
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P1001-A | MTC Bio | 100/pack | EUR 115.08 |
Description: Sealing film Aluminum membrane |
Sealing Mats for 96 Well Format |
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GP501 | GenDepot | 5 mats | EUR 176.4 |
Sealing Film, Foil Tight Seal, 100Sheet |
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SMT4406 | Bio Basic | 1PK, 100UNIT | EUR 247.92 |
SEALING TAPE APPLICATOR MANUAL; 2/PK |
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60180-M950 | Scientific Laboratory Supplies | PK2 | EUR 28.16 |
Nunc EASYPEAL-EASYSEAL SEALING TAPE ROLL |
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236707 | Scientific Laboratory Supplies | PK1000 | EUR 698.82 |
Benchmark Replacement Sealing Ring 100-2000mL |
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BOT1909 | Scientific Laboratory Supplies | PK10 | EUR 16.63 |
Sealing film for fluorescence measurement black |
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MOL2254 | Scientific Laboratory Supplies | PK50 | EUR 74.1 |
Sealing film for luminescence measurement white |
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MOL2256 | Scientific Laboratory Supplies | PK50 | EUR 61.45 |
Sealing Mat for DWP 96/2000 |
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E0030127960 | Scientific Laboratory Supplies | PK50 | EUR 158.46 |
Kinesis Jumbo Syringe Sealing CS Septa |
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CHR1737 | Scientific Laboratory Supplies | PK50 | EUR 53.58 |
Kinesis Sealing Ring; 1/16; PTFE |
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CHR1847 | Scientific Laboratory Supplies | PK20 | EUR 46.74 |
Hettich Sealing lid for 250ml bucket |
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CEN0622 | Scientific Laboratory Supplies | EACH | EUR 51.3 |
Greiner silverseal aluminum sealing tape textured |
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Z617601-100EA | Scientific Laboratory Supplies | PK100 | EUR 105.56 |
Weighted sealing flake, accessory for AgileSealer? |
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APS-200-SF | ACTGene | each | EUR 421.2 |
Cover Plate |
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1292235 | Atto | 5unit | EUR 301.2 |
Plate Rotator |
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abx725037-1Unitwith1215ml450mlplate | Abbexa | 1 Unit with 12 × 15 ml 4 × 50 ml plate | EUR 706.8 |
Plate Rotator |
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abx725037-1Unitwith1215ml450mlplate2215mlplate | Abbexa | 1 Unit with 12 × 15 ml 4 × 50 ml plate + 22 × 15 ml plate | EUR 748.8 |
dummy plate |
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EVS3100-DUMMY | Consort | ea | EUR 57.6 |
Plate Roller |
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TSM113 | Bio Basic | 1 UNIT | EUR 66.26 |
Plate Holder |
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FB02202 | Scientific Laboratory Supplies | EACH | EUR 31.92 |
Pierce Plate |
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PCR1380 | Scientific Laboratory Supplies | EACH | EUR 318.06 |
Androstenedione ELISA Kit, (1 Plate) |
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K070-H1 | Arbor Assays | 1x96 well plate | EUR 542 |
Androstenedione ELISA Kit, (5 Plate) |
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K070-H5 | Arbor Assays | 5x96 well plate | EUR 2224 |
Barcoding for CrystalDirect Plate, service per plate |
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M-XDIR-BAR-1 | MiTeGen | 1 Service | EUR 0.5 |
Description: Barcoding for CrystalDirect Plate, service per plate |
Pressure Sealing Mats for 384 Well Format |
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GP504 | GenDepot | 5 mats | EUR 235.2 |
Technical Observe: Utilizing enzyme-linked immunosorbent assays to judge humoral responses to vaccination in opposition to respiratory viruses in beef cattle
This experiment evaluated humoral responses in beef calves vaccinated in opposition to parainfluenza-Three virus (PI3), bovine respiratory syncytial virus (BRSV), and bovine herpesvirus-1 (BHV-1) utilizing by way of serum neutralization (SN) checks or enzyme-linked immunosorbent assays (ELISA). Blood samples have been collected from 50 overtly wholesome Angus-influenced steers (183 ± Three kg of physique weight, 212 ± 2 d of age) on d 0, 21, 35, and 49 of the experiment. Steers have been vaccinated in opposition to respiratory viruses on d Zero and 21.
Blood was processed for serum assortment and frozen in duplicates. One of many duplicates was analyzed for antibodies in opposition to BRSV, PI3, and BHV-1 utilizing commercially accessible ELISA (IDEXX Switzerland AG, Liebefeld-Bern, Switerland), and outcomes reported as pattern:constructive management (S/P, %) ratio. The opposite duplicate was analyzed for antibodies in opposition to the identical vaccine antigens by way of SN.
This methodology report outcomes as titers, the best dilution that gives full safety of the cells, which have been remodeled with base 2 log for statistical analyses. Samples have been categorized as constructive for presence of antibodies by SN if log-transformed titer ≥ 2 for all viruses, and by ELISA if S/P ratio ≥ 50% for BHV-1 or ≥ 20% for PI3 and BRSV. Day results have been detected (P < 0.01) for SN and ELISA throughout all vaccine antigens, as antibody ranges elevated after vaccine administration.
Linear suits have been detected (P < 0.01) throughout all vaccine antigens when regressing the SN and ELISA outcomes; as SN titer elevated the ELISA S/P ratio linearly elevated (P < 0.01). Kendall (τ) and Spearman’s rank (ρ) correlations have been additionally detected (P < 0.01) between SN and ELISA outcomes throughout all vaccine antigens. The SN and ELISA have been very strongly correlated (ρ ≥ 0.83) for BHV-1 and PI3, and strongly correlated (ρ = 0.66) for BRSV. Cohen’s kappa coefficient for analysis settlement between strategies have been robust for BHV-1 and PI3 (κ ≥ 0.88), however weak (κ = 0.47) for BRSV.
The sensitivity of the ELISA in yielding true constructive outcomes approached 100% throughout all antigens. The specificity of the ELISA in yielding unfavorable outcomes have been passable for BHV-1 and PI3 assays (84.Zero and 88.5%, respectively) however not for BRSV (34.4%).
Regardless of limitations in detecting true BRSV negatives, outcomes from this experiment point out that the business ELISAs examined herein can be utilized as surrogate for SN checks in quantifying humoral responses to vaccination in opposition to BHV-1, PI3, and BRSV in beef cattle.
Key phrases: beef cattle; humoral response; respiratory illness; vaccination
EZBlock? (TBS) Blocking Buffer |
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2117-1000 | Biovision | each | EUR 254.4 |
EZBlock? (TBS) Blocking Buffer |
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2117-200 | Biovision | each | EUR 150 |
EZBlock? (PBS) Blocking Buffer |
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2128-1000 | Biovision | each | EUR 274.8 |
EZBlock? (PBS) Blocking Buffer |
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2128-200 | Biovision | each | EUR 164.4 |
Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed |
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ELISA-1 | Alpha Diagnostics | 1 | EUR 242.4 |
Neptune Assay Diluent-AD3 |
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KF17349 | Neuromics | 500 ml | EUR 292.8 |
Neptune Sample Diluent-SD3 |
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KF17354 | Neuromics | 500 ml | EUR 292.8 |
Filter tip NEPTUNE BT10E |
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DD713002 | Scientific Laboratory Supplies | PK960 | EUR 116.28 |
Neptune 10ul barrier tip |
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BT10 | Biotix | 10 trays of 96 / pack | EUR 70.8 |
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface |
Neptune 100ul barrier tip |
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BT100 | Biotix | 10 trays of 96 / pack | EUR 70.8 |
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface |
Neptune 1000ul barrier tip |
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BT1000.96 | Biotix | 8 trays of 96 / pack | EUR 70.8 |
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface |
Neptune 20ul barrier tip |
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BT20 | Biotix | 10 trays of 96 / pack | EUR 70.8 |
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface |
Neptune 200ul barrier tip |
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BT200 | Biotix | 10 trays of 96 / pack | EUR 70.8 |
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface |
Neptune 300ul barrier tip |
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BT300 | Biotix | 10 trays of 96 / pack | EUR 70.8 |
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface |
TrueBlack WB Blocking Buffer Kit |
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23013 | Biotium | 1kit | EUR 416.4 |
Description: Minimum order quantity: 1 unit of 1kit |
TrueBlack WB Blocking Buffer Kit |
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23013-T | Biotium | 1kit | EUR 150 |
Description: Minimum order quantity: 1 unit of 1kit |
Blocking Buffer and Coating Stabilizer |
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abx090811-1000ml | Abbexa | 1000 ml | EUR 627.6 |
3 Blocking Buffer Sample Pack |
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KF17340 | Neuromics | 3 x 100 ml | EUR 246 |
10 x Universal Blocking Buffer |
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GR103005 | Genorise Scientific | 200 mL | EUR 154.8 |
TBS blocking buffer with BSA |
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SD8113 | Bio Basic | 1pk, 1L | EUR 100.72 |
PBS Blocking buffer with BSA |
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SB0626 | Bio Basic | 1pk, 1L | EUR 100.72 |
West-Ezier Super Blocking Buffer |
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W3700-010 | GenDepot | 100ml | EUR 116.4 |
West-Ezier Super Blocking Buffer |
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W3700-050 | GenDepot | 500ml | EUR 198 |
West-Ezier Super Blocking Buffer |
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W3700-100 | GenDepot | 1L | EUR 295.2 |
EZBlock? T20 (TBS) Blocking Buffer |
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2140-1000 | Biovision | each | EUR 261.6 |
EZBlock? T20 (TBS) Blocking Buffer |
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2140-200 | Biovision | each | EUR 144 |
EZBlock? T20 (PBS) Blocking Buffer |
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2143-1000 | Biovision | each | EUR 261.6 |
EZBlock? T20 (PBS) Blocking Buffer |
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2143-200 | Biovision | each | EUR 144 |
Block |
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BSH100-02 | Benchmark Scientific | 1 PC | EUR 164.65 |
Block |
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BSH100-05 | Benchmark Scientific | 1 PC | EUR 176.14 |
Block |
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BSH100-1213 | Benchmark Scientific | 1 PC | EUR 164.65 |
Block |
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BSH100-1232 | Benchmark Scientific | 1 PC | EUR 164.65 |
Block |
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BSH100-15 | Benchmark Scientific | 1 PC | EUR 164.65 |
Block |
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BSH100-150 | Benchmark Scientific | 1 PC | EUR 187.62 |
Block |
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BSH100-1520 | Benchmark Scientific | 1 PC | EUR 164.65 |
Block |
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BSH100-15HL | Benchmark Scientific | 1 PC | EUR 187.62 |
Block |
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BSH100-500 | Benchmark Scientific | 1 PC | EUR 124.98 |
Block |
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BSH100-5MT | Benchmark Scientific | 1 PC | EUR 187.62 |
Block |
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BSH100-CV | Benchmark Scientific | 1 PC | EUR 164.65 |
Block |
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BSW02 | Benchmark Scientific | 1 PC | EUR 182.32 |
Block |
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BSW05 | Benchmark Scientific | 1 PC | EUR 182.32 |
Block |
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BSW10 | Benchmark Scientific | 1 PC | EUR 182.32 |
Block |
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BSW13 | Benchmark Scientific | 1 PC | EUR 182.32 |
Block |
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BSW15 | Benchmark Scientific | 1 PC | EUR 182.32 |
Block |
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BSW1500 | Benchmark Scientific | 1 PC | EUR 182.32 |
Block |
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BSW1516 | Benchmark Scientific | 1 PC | EUR 219.64 |
Block |
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BSW1520 | Benchmark Scientific | 1 PC | EUR 182.32 |
Block |
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BSW50 | Benchmark Scientific | 1 PC | EUR 182.32 |
Block |
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BSW5MT | Benchmark Scientific | 1 PC | EUR 182.32 |
Block |
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BSWHEM | Benchmark Scientific | 1 PC | EUR 284.71 |
Block |
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BSWMT | Benchmark Scientific | 1 PC | EUR 233.99 |
Block |
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BSWNMR | Benchmark Scientific | 1 PC | EUR 578.08 |
Block |
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BSWPCR1 | Benchmark Scientific | 1 PC | EUR 306.72 |
Block |
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BSWPCR2 | Benchmark Scientific | 1 PC | EUR 250.26 |
Block |
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H5000-02 | Benchmark Scientific | 1 PC | EUR 348.4 |
Block |
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H5000-025 | Benchmark Scientific | 1 PC | EUR 709.62 |
Block |
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H5000-05 | Benchmark Scientific | 1 PC | EUR 348.4 |
Block |
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H5000-12 | Benchmark Scientific | 1 PC | EUR 459.06 |
Block |
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H5000-1232 | Benchmark Scientific | 1 PC | EUR 459.06 |
Block |
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H5000-15 | Benchmark Scientific | 1 PC | EUR 348.4 |
Block |
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H5000-150 | Benchmark Scientific | 1 PC | EUR 459.06 |
Block |
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H5000-20 | Benchmark Scientific | 1 PC | EUR 459.06 |
Block |
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H5000-500 | Benchmark Scientific | 1 PC | EUR 459.06 |
Block |
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H5000-CMB | Benchmark Scientific | 1 PC | EUR 348.4 |
Block |
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H5000-CU | Benchmark Scientific | 1 PC | EUR 459.06 |
Block |
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H5000-WB | Benchmark Scientific | 1 PC | EUR 5146.62 |
Neptune 1000-1250ul barrier tip |
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BT1250 | Biotix | 8 trays of 96 / pack | EUR 70.8 |
Description: Extended Length, Low retention, Racked, Pre-Sterile, Sample Saving Surface |
Suez neptune NCP 8 Cartridge |
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WAT3262 | Scientific Laboratory Supplies | EACH | EUR 451.44 |
ChonBlock Blocking/Sample Dilution ELISA Buffer, 100 ml |
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9068 | Chondrex | 100 ml | EUR 155.7 |
Description: ChonBlock Blocking/Sample Dilution ELISA Buffer |
ELISA Plate Blocking Buffer concentrate (10X) BSA-based |
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80060 | Alpha Diagnostics | 50 ml | EUR 242.4 |
Block 16mm For Block Heater |
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BLO5016 | Scientific Laboratory Supplies | EACH | EUR 133.38 |
Casein-based PBS Blocking Buffer Pack |
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AR0182 | BosterBio | 500mL/Pack | EUR 84 |
Casein-based TBS Blocking Buffer Pack |
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AR0183 | BosterBio | 500mL/Pack | EUR 84 |
Blocking Buffer for ICC and IHC |
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SF40011 | Neuromics | 10 ml | EUR 153.6 |
Corning 3D Clear Blocking Buffer - 30mL |
|||
5736 | Scientific Laboratory Supplies | EACH | EUR 108.3 |
Corning 3D Clear Blocking Buffer - 100mL |
|||
5737 | Scientific Laboratory Supplies | EACH | EUR 294.12 |
EZBlock? Blocking Buffer and Signal Enhancer |
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M4104-100 | Biovision | each | EUR 183.6 |
EZBlock? Blocking Buffer and Signal Enhancer |
|||
M4104-500 | Biovision | each | EUR 430.8 |
Block-Free ELISA Reagent |
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M4102-250 | Biovision | each | EUR 542.4 |
Block-Free ELISA Reagent |
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M4102-50 | Biovision | each | EUR 176.4 |
Corning 3D Clear Starter Kit - Includes Reagent/Antibody Buffer/Blocking Buffer/Penetration Buffer/Washing Buffers |
|||
5730 | Scientific Laboratory Supplies | EACH | EUR 864.12 |
Neptune 10ul extended length barrier tip |
|||
BT10XLS3 | Biotix | 10 trays of 96 / pack | EUR 70.8 |
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface |
Super Block |
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AAA125 | ScyTek Laboratories | 125 ml | EUR 88.8 |
Super Block |
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AAA500 | ScyTek Laboratories | 500 ml | EUR 121.2 |
Super Block |
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AAA999 | ScyTek Laboratories | 1000 ml | EUR 148.8 |
Peroxide Block |
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ACA015 | ScyTek Laboratories | 15 ml | EUR 80.4 |
Peroxide Block |
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ACA125 | ScyTek Laboratories | 125 ea. | EUR 91.2 |
Peroxide Block |
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ACA500 | ScyTek Laboratories | 500 ml | EUR 121.2 |
Peroxide Block |
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ACA999 | ScyTek Laboratories | 1000 ml | EUR 150 |
EZ Block |
|||
EZB125 | ScyTek Laboratories | 125 ml | EUR 111.6 |
EZ Block |
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EZB500 | ScyTek Laboratories | 500 ml | EUR 152.4 |
EZ Block |
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EZB999 | ScyTek Laboratories | 1000 ml | EUR 207.6 |
Pro-Block |
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PBK-20000 | ScyTek Laboratories | 20 L | EUR 1452 |
Pro-Block |
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PBK010 | ScyTek Laboratories | 10 L | EUR 811.2 |
Pro-Block |
|||
PBK125 | ScyTek Laboratories | 125 ml | EUR 88.8 |
Pro-Block |
|||
PBK500 | ScyTek Laboratories | 500 ml | EUR 121.2 |
Pro-Block |
|||
PBK999 | ScyTek Laboratories | 1000 ml | EUR 148.8 |
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