Enzyme-linked immunosorbent assays for serosurveys
Standardization of enzyme-linked immunosorbent assays for serosurveys of the SARS-CoV-2 pandemic utilizing scientific and at-home blood sampling
The extent of SARS-CoV-2 an infection all through the US inhabitants is at the moment unknown. Prime quality serology is a key instrument to understanding the unfold of an infection, immunity towards the virus, and correlates of safety.
Restricted validation and testing of serology assays used for serosurveys can result in unreliable or deceptive information, and scientific testing utilizing such unvalidated assays can result in medically pricey diagnostic errors and improperly knowledgeable public well being choices.
Estimating prevalence and scientific determination making is very depending on specificity. Right here, we current an optimized ELISA-based serology protocol from antigen manufacturing to information evaluation.
This protocol defines thresholds for IgG and IgM for willpower of seropositivity with estimated specificity nicely above 99%. Validation was carried out utilizing each historically collected serum and dried blood on mail-in blood sampling kits, utilizing archival (pre-2019) destructive controls and identified PCR-diagnosed optimistic affected person controls.
Minimal cross-reactivity was noticed for the spike proteins of MERS, SARS1, OC43 and HKU1 viruses and no cross reactivity was noticed with anti-influenza A H1N1 HAI titer throughout validation.
This technique is very particular and is designed to offer good estimates of seroprevalence of SARS-CoV-2 seropositivity in a inhabitants, offering particular and dependable information from serosurveys and scientific testing which can be utilized to higher consider and perceive SARS-CoV-2 immunity and correlates of safety.
Analytical validation of an enzyme-linked immunosorbent assay for the quantification of S100A12 within the serum and feces of cats.
Measuring S100A12 concentrations in serum and feces is a delicate and particular marker of irritation, similar to seen with power gastrointestinal irritation in individuals and canine. Biomarkers of irritation in cats are at the moment missing. We aimed to analytically cross-validate the canine S100A12-ELISA for the measurement of S100A12 in feline specimens.
The ELISA was analytically validated by assessing dilutional linearity, spiking/restoration, intra- and inter-assay variability. Reference intervals for serum and fecal feline S100A12 concentrations had been calculated utilizing samples from wholesome cats, and the short-term organic variation of fecal S100A12 was assessed.
Noticed-to-expected ratios (O/E) for serial dilutions of serum and fecal extracts ranged from 91%-159% (imply, 120%) and 100%-128% (imply, 114%), and for the spiking/restoration technique ranged from 106%-263% (imply, 154%) and 52%-171% (imply, 112%).
Intra- and inter-assay CV% for serum had been ≤5.6% and ≤14.0%, and for fecal extracts had been ≤3.8% and ≤19.1%, repsectively. RIs for feline serum and fecal S100A12 concentrations had been <43 µg/L and < 20 ng/g, respectively. A gentle short-term biologic variation, however giant individuality had been detected when measuring fecal S100A12 concentrations in wholesome cats.
elisareagents
The canine S100A12-ELISA is correct, reproducible, and sufficiently linear and exact for the measurement of S100A12 in feline serum and fecal samples. The usage of this assay is an inexpensive choice for the measurement of S100A12 concentrations in feline specimens and supplies a foundation for the additional analysis of S100A12 in cats with gastrointestinal illness. Utilizing a population-based RI for fecal feline S100A12 seems to be of restricted worth.
Description: A competitive ELISA for quantitative measurement of Rat Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: For the quantitative determination of human Cystatin C concentrations in cell culture supernates, serum, plasma, saliva, urine, and human milk.
Description: A competitive ELISA for quantitative measurement of Human Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Cystatin C,Cys-C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Cystatin C,Cys-C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Cystatin C,Cys-C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Quantitativecompetitive ELISA kit for measuring Pig Cystatin C, Cys-C in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativecompetitive ELISA kit for measuring Pig Cystatin C, Cys-C in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativesandwich ELISA kit for measuring Rat Cystatin C, Cys-C in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Rat Cystatin C, Cys-C in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A competitive ELISA for quantitative measurement of Canine Cystatin C,Cys-C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Cystatin C,Cys-C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Cystatin C,Cys-C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Cystatin C,Cys-C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Cystatin C,Cys-C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Cystatin C,Cys-C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Cystatin C,Cys-C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Cystatin C,Cys-C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Cystatin C,Cys-C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Analysis of Serotonin Launch Assay and Enzyme-Linked Immunosorbent Assay Optical Density Thresholds for Heparin-Induced Thrombocytopenia in Sufferers on Extracorporeal Membrane Oxygenation.
Heparin-induced thrombocytopenia is a acknowledged concern in sufferers on extracorporeal life assist. The aim of this research was to judge the applicability of an enzyme-linked immunosorbent assay optical density threshold lower than 1 to rule out heparin-induced thrombocytopenia in sufferers on extracorporeal membrane oxygenation.Retrospective, single-center research.
Sufferers had been recruited from a prospectively maintained database of all sufferers on extracorporeal membrane oxygenation from 2012 to 2018 at a tertiary referral middle.Forty-seven sufferers on extracorporeal membrane oxygenation assist.The first goal was to judge the appliance of enzyme-linked immunosorbent assay optical density thresholds and the serotonin launch assay in sufferers on extracorporeal membrane oxygenation.
Sufferers had been divided into two cohorts, serotonin launch assay destructive and serotonin launch assay optimistic. With a view to carry out a sensitivity and specificity evaluation of enzyme-linked immunosorbent assay optical density thresholds, heparin-induced thrombocytopenia destructive was outlined as an optical density lower than 1.Zero and heparin-induced thrombocytopenia optimistic as an optical density higher than or equal to 1.0.
Using the prespecified optical density thresholds, a specificity and destructive predictive worth of 89% and 95% had been achieved, respectively. This evaluation has helped to establish optical density thresholds for sufferers present process extracorporeal membrane oxygenation. ”
Our information recommend that an optical density threshold of 1.Zero might assist clinicians in objectively ruling out heparin-induced thrombocytopenia with out sending a confirmatory serotonin launch assay. Growing the optical density threshold to 1.Zero resulted in a excessive specificity and destructive predictive worth.
Description: For quantitative and direct determination of formaldehyde concentrations in biological, food and environmental samples. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 1.5 µM (45 ppb) formaldehyde can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL370/470nm. Samples: Biological, food, beverage, environment. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 1.5 µM (45 ppb).
Description: Purification Detection kit used to capture, detect, identify and characterise ubiquitinated proteins and free chains from samples.
in Cell Lysates, Tissue samples from all species
Description: The most affordable qPCR Detection Kit for SARS-CoV-2 on the market.abm’s GenomeCoV19 Detection Kit is a real-time reverse transcription-polymerase chain reaction (RT-qPCR) test intended for the qualitative detection of RNA from SARS-CoV-2 in human nasopharyngeal and oropharyngeal swab specimens from individuals suspected of COVID-19 by their healthcare provider. Our GenomeCoV19 Detection Kit is the most affordable qPCR detection kit on the market at only $1.77 USD/test (limited time pricing for US customers only).This kit is widely used in Europe under the CE-IVD certification and is listed by U.S. Food and Drug Administration (FDA) for distribution in the USA, under Section IV.C.
Description: The most affordable qPCR Detection Kit for SARS-CoV-2 on the market.abm’s GenomeCoV19 Detection Kit is a real-time reverse transcription-polymerase chain reaction (RT-qPCR) test intended for the qualitative detection of RNA from SARS-CoV-2 in human nasopharyngeal and oropharyngeal swab specimens from individuals suspected of COVID-19 by their healthcare provider. Our GenomeCoV19 Detection Kit is the most affordable qPCR detection kit on the market at only $1.77 USD/test (limited time pricing for US customers only).This kit is widely used in Europe under the CE-IVD certification and is listed by U.S. Food and Drug Administration (FDA) for distribution in the USA, under Section IV.C.
Description: Direct Fluorometric detection assay to measure the total GSH content in Whole Blood, Serum, EDTA Plasma, Heparin Plasma, Erythrocytes, Urine, Cell Lysates, Tissue samples from all species
Description: Indirect Colorimetric assay used for quantitative measuring Nitrate and Nitrite present in a variety of samples in Serum, Plasma, Urine, Saliva, Water, Buffer, Cell Lysates, Tissue Culture Media samples from all species
Description: Monkeypox is a viral zoonosis (a virus transmitted to humans from animals) with symptoms very similar to those seen in the past in smallpox patients, although it is clinically less severe. It is caused by the monkeypox virus which belongs to the orthopoxvirus genus of the Poxviridae family. There are two clades of monkeypox virus: the West African clade and the Congo Basin (Central African) clade. The name monkeypox originates from the initial discovery of the virus in monkeys in a Danish laboratory in 1958. The first human case was identified in a child in the Democratic Republic of the Congo in 1970.
Description: Direct Colorimetric assay used for quantitative measuring H2O2 in a variety of samples in Urine, Buffer, Tissue Culture Media samples from all species
Description: Purification Detection kit used to capture, detect, identify and characterise isolated ubiquitin binding proteins by Western blotting or proteomic analysis in samples.
in Cell Lysates, Tissue samples from all species
Description: The SARS-CoV-2 IgG detection kit is designed for qualitative detection of human IgG antibodies in serum collected from individuals suspected of prior infection with the virus that causes COVID-19. This fast and simple ELISA uses the receptor binding domain (RBD) of the SARS-CoV-2 Spike protein (BPS Bioscience #100696) to identify IgG antibodies that indicate a previous infection with SARS-CoV-2.
Description: The SARS-CoV-2 IgG detection kit is designed for qualitative detection of human IgG antibodies in serum collected from individuals suspected of prior infection with the virus that causes COVID-19. This fast and simple ELISA uses the trimeric form of the SARS-CoV-2 Spike protein (BPS Bioscience #100728) to identify IgG antibodies that indicate a previous infection with SARS-CoV-2. The Spike protein is expressed on the viral membrane as a trimer, which means this kit measures IgG antibodies in a more physiologically relevant context than many other commercially available ELISA kits.
Description: Direct Colorimetric assay used for quantitative measuring urea nitrogen in a variety of samples in Serum, Plasma, Urine, Saliva, Tissue Culture Media samples from all species
Description: Western blot detection of IP proteins can exhibit high background due to the use of the same primary antibody in the IP and the Western blot. The Pure-IP Western Blot Detection Kit eliminates this background problem by using a proprietary HRP Conjugate for detection of the primary antibody.
Description: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules to activate various kinases and transcription factors so the organism can respond to potential infection. These adaptor molecules include MyD88, TIRAP, TIRP, TOLLIP, and TRIF. These molecules interact with and activate the IL-1R-associated kinase (IRAK) family, which then activates TNF receptor associated factor (TRAF)-6, and ultimately leads to the activation of NF-κB. While most TLRs utilize more than one adaptor, certain adaptor molecules are essential for individual TLR signaling, e.g., TLR4 signaling is dependent on TIRP expression.;;For images please see PDF data sheet
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